FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349180
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA614882.617423952187572No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT520522.2157518793791877No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA310871.3233128155356337No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC249791.063307196553691No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA207980.8853302003252199No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTA167310.7122059612290246No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA151710.6457998109978801No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT148570.6324334448616113No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC145110.6177049012847037No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA137320.5845443942141513No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC110820.4717390749112456No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA107800.45888352531521637No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT105740.4501145080411037No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA100730.4287879174861015No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT97570.4153364152597928No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG86980.37025685558365046No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA84800.3609770217692982No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC84320.3589337556083399No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC80880.3442903481214722No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC78690.33496794626209997No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA76630.3261989289879873No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT66610.283545747877983No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA64270.2735848253433113No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGTGAT62160.2646029678440988No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61680.2625597016831405No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTG58420.2486825190066321No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT56360.23991350173251944No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT55550.2364654900859023No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA50280.21403213036038107No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA50080.21318076945998177No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC49610.21118007134404343No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA49580.21105236720898357No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC49500.21071182284882387No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACCG48570.20675299466196714No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA45210.1924501315352591No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG43170.18376625035118638No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC41660.17733847555317175No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT41060.17478439285197386No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA39550.16835661805395924No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA39130.16656876016312075No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGTGAC38680.16465319813722235No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38330.16316331656152358No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT37560.15988557709498633No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC37310.15882137596948723No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT36730.15635242935832927No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA36480.15528822823283017No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35850.15260644139657242No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA34720.14779625230931645No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC34130.14528473765313854No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT34070.14502932938301874No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA33520.14268808690692072No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC33050.1406873887909824No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC31140.13255689219216918No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA30950.13174809933678985No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT30570.1301305136260312No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC30230.12868320009535242No Hit
CTGACCTATGAATTGACAGCTTATTCCATAGCATG30090.12808724746507294No Hit
TCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29690.12638452566427436No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA29020.12353246664793673No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28910.12306421815271712No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA28560.12157433657701837No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA28360.12072297567661908No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG28250.12025472718139947No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT28190.11999931891127967No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC27600.11748780425510179No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC26770.11395465651844473No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG26210.11157084599732674No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGTGGC25500.10854851480090925No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATA25340.10786742608058983No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA24490.10424914225389285No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA24210.10305723699333386No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC24170.10288696481325398No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG23540.10020517797699623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)