FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244744
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT51574515.894782454332299No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2584977.966637737830781No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC649672.00222267149581No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC409341.2615479063987791No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT346261.0671411981962213No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA329521.0155500711304188No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA310800.9578567677450054No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA301210.9283012773889096No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA292810.9024132566390446No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG289750.8929826205087366No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN226120.6968808633285091No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT192300.5926507607379812No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG182900.5636808327559893No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA167140.5151099747776712No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA165240.5092543510366303No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA150000.46228608481901806No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT142540.43929505686735226No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA131070.403945580914858No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG128060.3946690401461564No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA114850.3539570456097615No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT110120.3393796244018018No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA88360.2723173230307229No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATAC87330.26914295858163234No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA82870.25539765232634687No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT78530.2420221749389166No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG78340.2414366125648125No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA76970.2372143996567988No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGAC76840.23681375171662233No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC74650.23006437487826467No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA74630.23000273673362215No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC73350.22605789547649985No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC70740.21801411760064893No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG70370.216873811924762No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG68170.2100936160140831No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA66270.2042379922730422No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT65580.20211147628287468No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC62510.19265002108024548No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT62500.1926192020079242No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGCA61280.18885927518472953No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA60650.18691767362848966No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA60450.1863012921820643No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC59700.1839898617579692No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC53540.1650053132080682No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA52190.16084473844469702No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA50480.1555746770777602No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC48400.14916431003493652No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA47940.1477466327081582No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC46600.14361687701710826No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATCA45670.14075070329123035No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42080.1296866563278952No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT42030.12953256096628887No Hit
GNNACTGGGGAAACTTCCGAAAGATAAGGTTAATG42020.1295017418939676No Hit
GNNGTCAAAAGGGTCCTTAGGGAGCGGAACCGGCA40830.12583427228773672No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC40830.12583427228773672No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA40050.12343038464667784No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG39920.12302973670650133No Hit
GNNCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT39310.12114977329490399No Hit
GNNTGACCCCTTTGAAGGCTTTCTATTCCAATTAC39220.1208724016440126No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38970.12010192483598091No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37560.11575643563868214No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATCGT37310.11498595883065042No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA36420.11224306139405758No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC36400.11218142324941505No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC36110.11128767015209828No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC34360.1058943324958764No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTGTTAGCA32450.10000788968251426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)