FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753544
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT633941.6889105336183616No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT544991.4519344917763053No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC544021.4493502673739804No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA535241.425959040309638No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA342250.9118049502017294No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA297090.7914919873058636No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA261710.6972344003427161No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT252000.6713655148307839No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCGT238630.6357458444605951No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA210490.5607766953044909No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT210230.5600840165987131No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCTAC204610.5451114999584392No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA189240.5041635318514982No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA187960.5007534212999768No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA182140.48524807488602767No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA179420.4780015899640447No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178630.47589691235802756No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCATG157030.41835129680110317No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA147700.3934947878591539No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC144590.38520928487850414No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG113590.30262066995884424No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA111720.29763871157498084No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG109070.29057871707378413No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC108110.288021134160143No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT95160.2535204063146722No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT90020.23982668113121894No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCAAC87700.23364585575658633No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCTTG84980.22639937083460326No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA83820.22330895814728696No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT80260.2138245881758679No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT79310.2112936467509106No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC78730.20974844040725246No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC76310.20330120014578223No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA73300.19528211205197007No Hit
ATCCCCGGCATCTCCACCACGCCTTGGCCGTACAG72290.1925913216949102No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT71070.1893410600754913No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT71010.18918121114338876No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCCA66140.17620680615439702No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA65650.17490137320889274No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC61960.1650706638845848No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC61390.1635520990296104No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC61300.16331232563145656No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATCGT61050.16264628841436252No Hit
TACCCTGTAGATCCGATAAACAGCGGAACCGGCAT60490.1611543650480719No Hit
ATCCCCGGCACCTCCACCACGCCTTGGCCGTACAG58730.1564654630397299No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATGCA58240.1551600300942256No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATCGT56400.15025799617641356No Hit
ATCACATTGCCAGGGATTTCCATCGCCTTGGCCGT56190.14969852491405455No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC55130.14687452711357588No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGATG54760.1458887920322767No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATTAC54310.14468992504150746No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAGCA53930.1436775484715245No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA53620.1428516623223279No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51620.13752336458557568No Hit
TAGCTTATCAGACTGATGTTCTGGCGACCAATCGT50800.13533876251350724No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA49620.1321950668488234No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC49530.13195529345066956No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG49130.1308896339033191No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATGGT48300.12867839034256692No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC47690.12705325953285748No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA45060.12004654800902825No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA44840.11946043525798553No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT44140.11759553105012224No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCTAC43700.11642330554803675No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC43630.11623681512725041No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG43260.11525108004595123No Hit
AACCCGTAGATCCGATGAACACGCGGAACCGGCAT43240.11519779706858373No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC43100.11482481622701107No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG43000.11455840134017345No Hit
TTTGTTCGTTCGGCTCGCGTGATATTCCAATTACG42320.11274678010967769No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT41240.10986949933183146No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT40180.10704550153135277No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40010.10659259622372882No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA39640.10560686114242966No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT39640.10560686114242966No Hit
TAGCTTATCAGACTGATGTTCTGGCGACCAATTAC39480.10518059732348949No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT39350.10483425797060059No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATCCA38880.10358210800246381No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA38220.10182376974933556No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA37920.10102452508882272No Hit
TAGCTTATCAGACTGATGTTCTGTGTACTTGGCCG37780.10065154424725006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)