FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653102
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1347973.6899325559483422No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT756742.071499782924211No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN594321.6268913378274135No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC562401.539513542189624No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN494411.3533977425212875No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT452281.2380710968377011No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC401421.098846952535133No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA398821.09172971354208No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT358800.9821789810413178No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA355450.9730086923387301No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA351230.9614568659730826No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA327310.8959782672369947No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT313700.8587222585079749No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA309310.846705074208166No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA301150.8243678933684304No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN293410.8031804203660342No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT290740.7958715634000912No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA286920.7854146968795286No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT274860.7524016575502135No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA249610.6832823173292177No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC241830.6619853483423129No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN239390.655306093287294No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC200400.5485748823876256No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA156630.4287589013391906No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA150930.4131557235467282No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC146130.40001620540570726No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA145650.39870225359160516No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC140240.3838929216868294No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG135990.37225897333280045No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC129720.3550954777610918No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG126990.34762237681838615No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA123860.3390543160305954No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG123670.33853421010417994No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCGCCT120810.3307052472118216No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT117000.3202757546873862No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA112160.30702674056185675No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC109440.2995810136152782No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA106620.29186154670742837No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT106050.2903012289281821No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT106050.2903012289281821No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC102820.2814594281791201No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA100280.27450643316282985No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA96440.26399481865001306No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGATC93260.25528988788158663No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA92590.25345583014106915No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT92470.2531273421875436No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA88640.24264310167085396No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA79490.21759589521453274No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA79470.21754114722227852No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACGT79460.21751377322615137No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT77140.21116300612465788No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC77010.21080714417500523No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT75030.2053870929418341No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC74020.20262231933299424No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG69770.19098837097896526No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA69760.19096099698283814No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG69350.18983866314162592No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC66210.18124322835770806No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCTTG65170.17839633276048683No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC65030.17801309681470706No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA64440.1763980310432066No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT63820.1747008432833247No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG60420.1653936846001015No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC60300.16506519664657598No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT59030.16158869913843082No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA57990.15874180354120965No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT57640.1577837136767602No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT56740.15532005402531876No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA56170.15375973624607253No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA54610.1494893928502407No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT53480.14639613128787535No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC52110.14264589381845896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC51690.14149618598111963No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA51630.14133194200435684No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCAAC51250.14029173015152602No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGCC50630.13859454239164415No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA50170.1373353385697963No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG49930.13667836266274525No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG49540.1356107768137873No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC48910.1338862150577783No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCGCC48740.13342085712361715No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC47830.1309298234760486No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC47460.1299169856193449No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA46840.128219797859463No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC46330.1268237240569795No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA45460.12444218639391946No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT45120.12351147052559715No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT44810.12266287664565621No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGAGA44400.12154054280444401No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG43060.11787242732340898No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC42960.1175986873621377No Hit
CGTACCGTGAGTAATAATGCGACGCCTTGGCCGTA42960.1175986873621377No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC42750.11702383344346803No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA42520.11639423153254412No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT41140.11261662006700059No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC40910.11198701815607667No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG39930.10930436653561822No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC38750.10607423499261724No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACCA38600.10566362505071032No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG38300.10484240516689652No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG38200.10456866520562524No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT37960.10391168929857421No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT36970.10120166368198863No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC36860.10090054972459023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)