FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476414
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1948325.6043957940567495No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1254383.608258395001286No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA839592.4151036096391283No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA828302.3826276156982455No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA518961.4928026408822423No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC508121.4616210842552124No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA477301.3729665108931215No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN415951.1964915571045336No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA364721.0491270602408114No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN361751.0405837739693833No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT348481.0024122558475486No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA340350.9790260883772762No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA311910.897217650141784No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT305890.8799009554097988No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC240410.691545943607407No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT212570.6114634217903852No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN197280.5674813183930337No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC188540.5423404692306497No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC185030.5322438581825986No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC184830.5316685527097751No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC171200.49246148473685813No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163830.4712614780633147No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA159860.4598416644277696No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC150120.43182428790126837No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC137510.3955512778397509No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA136030.39129401734085756No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA126360.3634779977298446No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA124100.3569770458869398No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT123220.3544457018065167No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA122960.35369780469184625No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT117040.33666876269627266No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT113460.3263707947327332No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC113310.3259393156281156No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT107460.30911163054803026No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA100510.28911976536741596No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC86500.24881961699613453No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA84400.2427789095314885No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA82920.23852164903259507No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA77730.22359247201282703No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA72720.20918106991860003No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA71850.20667849111181807No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA69670.2004076614580427No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA68840.19802014374582544No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT67030.1928136292167734No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT66670.1917780793656912No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT64480.1854784844382746No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC63280.18202665160133402No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC62390.17946654224726974No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG61640.17730914672418185No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT59680.17167115309051223No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC58370.16790290224351875No Hit
TCCCTGAGACCCTTTAACCTGTCGCAGGTTAATGC57720.16603315945684258No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAT56620.1628689793563137No Hit
AACCGGGCGGAAACACCACGCCTTGGCCGTACAGC55730.16030887000224944No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG55370.15927332015116727No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54880.15786382174274985No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG54080.15556259985145612No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC53470.15380791815934466No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT51780.14894658691398666No Hit
ATCCCGGACGAGCCCCCACGCCTTGGCCGTACAGC49760.14313600163846998No Hit
TGACCTATGAATGTCACTAACATAAGGTTAATGCA49300.1418127990509761No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA49130.14132378939907617No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT48170.13856232312952368No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC46420.13352840024231866No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC45800.13174495327656602No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT44910.12918484392250174No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA44720.1286383037233195No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT44630.12837941626054894No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA44330.1275164580513138No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC44270.12734386640946677No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA43990.12653843874751397No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43800.1259918985483317No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC43600.12541659307550826No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC43310.1245824001399143No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG41200.11851292740162708No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGTCTA41000.11793762192880365No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAATTAC38670.11123531317041066No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA38220.10994087585655793No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA38110.10962445784650506No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC37400.10758212341798186No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA37330.10738076650249366No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC36950.10628768610412914No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA36460.10487818769571174No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA35910.1032960976454473No Hit
ATCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGC35710.10272079217262385No Hit
CCGGGCGGAAACACCACGCCTTGGCCGTACAGCAG35590.10237560888892981No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC35120.10102364102779474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)