FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4733100
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2716385.7391138999809845No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1804293.8120682005450974No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1738533.67313177410154No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1178732.490397413957026No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC601441.2707105279837738No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN499881.056136570112611No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA494391.0445374067735733No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT462110.9763368616762799No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN435110.9192917960744543No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA423470.8946990344594452No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA389970.8239208975090321No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA387360.8184065411675224No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA361760.764319367856162No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT334230.7061545287443748No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN327510.6919566457501426No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC289600.6118611480847648No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC287050.6064735585557034No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA275600.5822822251801145No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN273950.5787961378377807No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA272810.5763875684012593No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA261130.551710295577951No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA226960.47951659588852974No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA219930.46466375102998037No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGCTTG199780.42209122984935876No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC155860.329297923137056No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT149670.3162198136527857No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA139880.2955356954216053No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC139710.29517652278633455No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC135360.28598592888381824No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA127410.26918932623439185No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC125410.2649637658194418No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA122310.25841414717626926No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGTCCA120070.25368151951152523No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC115950.24497686505672817No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG115660.2443641587965604No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGT113750.24032874860028314No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT112470.2376243899347151No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT108740.22974371976083327No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT101870.21522891973547992No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA96360.20358750079229257No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG95220.20117893135577106No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA94250.1991295345545203No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG86790.18336819420675668No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA86450.18264984893621516No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC85780.1812342861972069No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC85220.18005112928102088No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGCTCA84500.17852992753163888No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG81970.17318459360672708No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA79240.1674167036403203No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA77740.16424753332910777No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC77200.16310663201707126No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA75870.1602966343411295No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA73270.15480340580169447No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA73010.15425408294775095No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA70530.1490143880332129No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGAC70010.1479157423253259No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA69230.1462677737634954No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT68420.14455642179544062No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC67320.1422323635672181No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC65320.13800680315226807No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA64340.13593627854894255No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA64050.1353235722887748No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA63070.1332530476854493No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGTCTG61170.12923876529124675No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT60990.12885846485390126No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC59170.12501320487629672No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT58530.1236610255435127No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA58390.12336523631446622No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC57980.12249899642940146No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC56940.12030170501362743No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT56920.12025944940947794No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT56290.11892839787876867No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT55340.1169212566816674No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT53420.11286471868331538No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA51950.1097589317783271No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA50590.10688555069616108No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC50590.10688555069616108No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA50430.10654750586296508No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA49970.10557562696752658No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC49870.10536434894677907No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC49120.1037797637911728No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA48510.10249096786461305No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT48490.10244871226046354No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC48220.10187826160444528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)