FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371683
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC781422.317596286483634No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA605981.797262672677117No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN420441.2469736923666905No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA407871.209692607519746No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT379211.1246905477175642No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT328090.9730748709175804No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA310970.9222990417545185No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN308460.9148546882966162No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA279930.8302381926177519No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGAGC270540.8023885994027316No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC241410.7159925770008627No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT234020.6940747395291906No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN208080.6171398675379626No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG196970.5841889643836624No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG194350.576418364359876No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN168180.4988013404581629No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC167190.4958651213652054No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT164800.4887766732519042No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA161960.48035358009634954No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC161290.47836644192232786No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA156350.4637150052362574No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC148420.4401955937138812No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC133380.39558879052390156No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC126450.37503525687319955No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC118310.35089301099777176No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT118300.350863352219055No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGAGA108080.32055208037054495No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT100120.29694369251201846No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGGAT95760.28401246499151905No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG94370.27988989474989195No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA87970.26090827637117725No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA85320.2530487000112407No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA83650.2480956839655448No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA81990.2431723266985657No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT81940.24302403280498197No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA75270.22324162740091522No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC67650.20064163801875798No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG67180.19924767541907112No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC66850.19826893572141863No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA66580.1974681486960666No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA64200.1904093593614821No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG63130.18723587003879072No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC61580.18263875933769574No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGTCG60990.18088889139340797No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG60560.1796135639085881No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA58140.17243613945913658No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT55130.1635088470653973No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA54820.1625894249251783No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC53450.1585261722409847No Hit
CCACTACGCCTTGGCCGTACAGCAGACCACTCCCT52910.15692459819028065No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAT50930.15105216000436578No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACGCC50600.15007342030671328No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC48660.1443196172356654No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC48340.14337053631672966No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC47890.14203589127447627No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT46390.137587074466965No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT43060.12771070115429No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA42890.12720650191610539No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA42500.12604980954615247No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA41910.12429994160186471No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAG41160.12207553319810908No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGCTA41090.12186792174709188No Hit
GGGCCGCCGGTGAAATACCACTACTCGCCTTGGCC39690.11771569272674805No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGGAG39650.11759705761188108No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC38740.11489810874865758No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA38170.1132075583618033No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT37970.11261438278746846No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA37900.11240677133645127No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG37710.11184325454083317No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT37620.11157632553238249No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA37110.11006372781782867No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT36990.10970782247322775No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA36890.10941123468606033No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA36860.10932225834991013No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC36440.10807658964380697No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA36110.10709784994615448No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT35790.10614876902721876No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA35430.10508105299341605No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA34390.1019965400068749No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC34040.10095848275178895No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA34020.10089916519435546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)