FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640693
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2436926.693560813834069No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1074472.951278781264995No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT899142.469694643300053No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT668551.8363262159154865No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA649761.7847151627451148No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN627631.7239300320021491No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA525701.4439558622493025No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA509241.3987446895412494No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN483951.3292798925918774No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA401251.1021253371267503No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA391391.075042581178913No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC354290.9731389051480034No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA341700.9385575768129859No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA308380.8470365394720181No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277750.7629042053257442No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA250350.6876438084727275No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT227400.6246063592837957No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN213580.5866465532798288No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA203350.5585475073014945No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA203260.5583003016184007No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGATG182020.49995976040825196No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC167980.4613956738456113No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA164430.4516447830124649No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT152490.4188488290553474No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC141020.3873438381099423No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA128390.35265264058243856No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA119860.3292230352847658No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC107310.29475157614223446No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA95830.2632191178987078No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA93660.2572587142063338No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA90460.24846917880744132No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT89030.24454135517606124No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC87250.23965217611042733No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA86310.23707025008700267No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT80370.22075467500280851No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT79930.2195461138854608No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT76650.21053684010159604No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA75880.20842185814623754No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA75810.2082295870593868No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC73370.20152756631773125No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA72840.2000717995172897No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC72610.19944005166049433No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC68330.18768404806447564No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA65610.18021294297541704No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA64400.17688939990271083No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC63180.17353838953188308No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA62970.17296157627133074No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61480.16886894885122145No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT60380.16584754605785218No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA58280.16007941345232898No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT57540.15804683339133513No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA55430.1522512334876904No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC55130.15142721454404423No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG54940.15090533587973498No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG54680.15019118612857496No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC53260.14629082979531643No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC52790.1449998667836041No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC52220.14343423079067638No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA52010.14285741753012407No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA51950.14269261374139483No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC50720.13931413607244553No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA49830.13686954653962857No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC48770.13395801293874546No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA48340.13277691911951928No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAC47970.13176062908902234No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA47290.1298928528167577No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT47220.1297005817299069No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT45490.12494873915488067No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA44430.12203720555399755No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG43650.11989475630051752No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA42200.11591199807289437No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41600.11426396018560203No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC41160.11305539906825432No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA39830.10940224841808964No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA38450.10561176127731725No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA38080.10459547124682031No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAGGT37970.1042933309674834No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG37910.10412852717875415No Hit
TCAATCCCCGGCACCTCCACCACGCCTTGGCCGTA37870.104018657986268No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAGGA37840.10393625609190338No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA37730.10363411581256646No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC37720.10360664851444491No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT36420.10003589975864485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)