FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803394
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1692724.45055127078604No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1353633.559005456705248No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT898872.3633365357362397No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA745751.9607487417816822No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC363240.9550417337777786No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG276190.7261672074994071No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT273840.7199885155206113No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC238980.6283335357840918No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA228050.5995960450061183No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT208350.5478002016094047No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA198170.5210346338033872No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT185860.4886688047570144No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT183500.4824638204719259No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA179880.4729460055939511No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA173630.45651331416098356No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC140360.36903881112501097No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA138050.3629652883713862No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA131440.3455860739118798No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA112540.295893615018586No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA107640.2830103849351395No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA98170.25811157087590714No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT92810.2440188947029942No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA91570.24075864872269348No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA90540.23805054117454041No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG85170.22393157269533473No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC84020.22090795747166872No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC80040.21044361956715502No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA79530.20910271194622487No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT78380.20607909672255886No Hit
CGCCTTGCGGCATGTCGTCGCCCGAGGGCTCCCGA72860.19156574364896195No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA72770.1913291128923272No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC71290.1874378515610005No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA70430.1851767132198242No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA69450.1826000672031349No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC65620.1725301138930124No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA63660.1673768218596338No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT62680.16480017584294449No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA61470.16161880678152196No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60120.158069345432001No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC59880.15743833008097505No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA59480.15638663782926512No Hit
GTCCAGTTTTCCCATTCCGAAAATAAGGTTAATGC58290.1532578533804281No Hit
CGCCTTGGCCGTACAGCGATAAATCTTTAGAAATC57840.15207469959725445No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC57760.15186436114691249No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55940.14707916140163235No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGTC55600.14618522298767891No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT54180.14245171549410868No Hit
CTGACCTATGAATTGACAGCCCGAAGGTCGGCATG51710.13595751583979993No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA50370.13243434679657168No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGGCGTAACT48970.12875342391558697No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48180.12667633171845988No Hit
CTGACCTATGAATTGACAGCCCGAAGGTCGGCAAC48160.12662374710587437No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG47890.1259138548359702No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA47660.12530913179123698No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT47400.12462553182762554No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC47080.1237841780262576No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46340.12183854736059424No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC46340.12183854736059424No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT45920.12073427049629883No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC45730.12023471667673663No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA45630.11997179361380914No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA45000.11831537831736602No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43890.11539693231887099No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT42480.11168971713159351No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA41990.11040139412324887No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG41260.10848205576387826No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA41230.10840317884500002No Hit
CGCCTTGCGGCATGTCGTCGCCCGAGGGCTCCCTC40560.10664159432338591No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG39230.10314471758645041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)