FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887253
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3660617.490117659143081No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1849853.785050620461024No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1045102.138420089976926No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA738521.5111147305040273No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT689421.4106492952175793No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA670931.3728161811962671No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN642961.3155856674495878No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA583321.1935539248735436No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA544701.114532028523999No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN537871.100556897709204No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA534341.0933340262924796No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC494141.0110792299887075No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT318970.652657024303837No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA284790.5827199860535152No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT247770.5069719124424293No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246120.5035957827433939No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA237850.48667421146398604No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGATG233420.47760981475687875No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA229680.4699572541057318No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA209970.4296278502463449No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA202120.4135656574357824No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC198060.4052583322369438No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA193940.39682823868541284No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN173760.35553714939660375No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT172720.3534091646166057No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA168810.3454087602994975No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT158090.3234741479518249No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA157360.3219804663274031No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA152990.3130388379729881No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA152010.3110336215456822No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC146450.29965708752953857No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA146310.2993706280399235No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT142380.29132930093858456No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA134820.2758604884993676No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAC125450.25668816408726947No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT120430.24641654524535564No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT111190.2275102189307572No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT109140.22331563354710715No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT105980.21684983363865143No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA105040.21492646277980698No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC100270.20516637874077726No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA95670.1957541383677088No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA95060.19450599344867148No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC93080.19045463780982896No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA91530.1872831220319472No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA86580.1771547329348409No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT85370.1746789044888816No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA79270.16219745529850818No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC72340.14801771056255938No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT71930.14717879348582935No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT71690.14668772007506056No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC69940.14310697645487147No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG69670.14255451886775658No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC66880.13684579046756942No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA65130.13326504684738033No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT65060.13312181710257276No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA62950.12880446336623047No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA62870.12864077222930753No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA62600.12808831464219267No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC62260.1273926273102702No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC61300.12542833366719505No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC59940.12264558433950523No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA59600.12194989700758278No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC58720.12014929450143055No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC57960.11859422870066272No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA57360.11736654517374075No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC57280.11720285403681782No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA55520.11360164902451336No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAGGT54640.11180104651836112No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC54150.11079843830470819No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC54110.11071659273624672No Hit
TAGCACCATCTGAAGCTAACCTATTCCAATTACGT52510.10744276999778812No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAG52380.10717677190028836No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG52300.10701308076336542No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA51540.1054580149625976No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)