FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187740
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT807342.5326406796037317No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT556041.744307879563578No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA537971.6876219516020756No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT528381.6575379422412118No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN488011.5308964972049164No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA464741.4578980719883052No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA441051.3835820989164738No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA399111.2520155345166168No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA362311.1365732462496942No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA337531.0588379227916955No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA304750.9560064497104531No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211860.6646087823975607No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211060.6620991674352362No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT207080.6496138329976723No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA190010.5960649237390753No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128970.40458130211372323No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA126960.3982758945208832No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC126460.3967073851694303No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT119000.3733052256457553No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC114390.3588435694253609No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA104370.32741064202224773No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT97310.30526328997973484No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC97030.30438492474292134No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA94900.2977030749057326No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC93230.2924642536718804No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC92550.29033108095390464No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAC89420.2805122124138104No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTTAATG87630.27489694893560956No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG85940.2695953873276992No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA84880.2662701475026194No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG79480.24933024650692967No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA78120.24506390107097817No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC74620.2340843356108089No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT71300.22366943351716262No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC70690.22175585210839027No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT70140.22003049182179224No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA67390.2114036903888021No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA64260.20158482184870785No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA63570.19942027894370307No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT56180.17623771072923136No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC54260.1702146348196528No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC51900.1628112706807958No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC51840.16262304955862147No Hit
ACAGCAGGCACAGACAGGCAGACGCCTTGGCCGTA48790.15305514251475968No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46230.14502437463532158No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA45490.14270298079517152No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGTCTG45350.1422637981767647No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA44350.13912677947385924No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG43750.13724456825211592No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT43630.13686812600776727No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC42110.1320998575793509No Hit
TCTCACACAGAAATCGCACCCGTCCGCCTTGGCCG41970.13166067496094414No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA41030.12871187738021295No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG40480.12698651709361491No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA40410.12676692578441154No Hit
CACGCTCATGCACACACCCACCGCCTTGGCCGTAC40290.12639048354006285No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39470.12381812820368035No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT37870.11879889827903153No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC36630.11490899508742872No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT35720.11205430806778471No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA34650.1086976980556758No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTATTAC34550.10838399618538525No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC33800.10603123215820612No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT33450.1049332756121892No Hit
CCCAGTGTTCAGACTACCTGTTCCGCCTTGGCCGT33410.10480779486407299No Hit
AGCAGCATTGTACAGGGCTATGTCGCAGGTTAATG33140.10396079981428849No Hit
CAACGGAATCCCAAAAGCAGCTCGCGAACCGGCAT32440.10176488672225463No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT32000.10038459849297621No Hit
AACTGGCCCTCAAAGTCCCGCTCGCCTTGGCCGTA31950.10022774755783094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)