FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4196441
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2051394.888404245407001No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1250062.9788575604899488No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1180372.8127882651036917No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA568201.3540045004802879No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN512531.221344467847874No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA507311.2089053557526486No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT497671.185933508894799No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT480351.1446604396439746No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA392980.936460205207222No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA364360.8682595561333998No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA363020.8650663741012921No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN355280.8466221734083715No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA308580.735337396617753No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA281890.671735882858832No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT273970.6528627472660762No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC262980.6266738886594616No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC253480.6040356578348177No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA250850.5977684423538899No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN203970.486054730663436No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200600.4780241161498518No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT196960.4693500992865145No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA178330.42495533715355466No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA173260.41287367080819193No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA167470.39907626486348785No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA152210.36271211724411234No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA120020.2860042593235554No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA117170.2792127900761622No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA116980.27876002545966927No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT116930.27864087687638167No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA116230.27697279671035524No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC106700.2542630767357387No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT99450.23698653215903667No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC98450.23460356049328468No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA98100.23376952041027146No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGCTTG96080.22895591764545242No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC92380.22013892248217No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT90800.21637382725028187No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT88380.210607035819162No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA88250.21029724950261422No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA87300.20803342642014985No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC84640.20169472178924952No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA82880.19750069165752598No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA82310.19614239780804732No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG76120.18139180319704246No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT76020.18115350603046726No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA74650.177888834848387No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG72360.1724318297338149No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT71390.17012034721803548No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA68220.16256632703760163No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC66710.1589680398223161No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC66420.158276978039248No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA65610.1563467709899889No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC65040.15498847714051026No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA64740.15427358564078464No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT64440.15355869414105905No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC62110.1480063701598569No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC60520.1442174452113112No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGT58040.13830767548024622No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA57010.1358532146645217No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA56150.13380385903197498No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT54980.1310157821830451No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT53580.1276796218509923No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA53120.12658345488474637No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT51820.12348559171926879No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG51780.1233902728526387No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA50990.1215077252366946No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA50150.11950602903746295No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC48800.11628901728869773No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA48500.11557412578897214No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA46610.11107030934070085No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC46430.11064137444086548No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA45690.108877975408209No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA45480.10837755135840108No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT44680.10647117402579948No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA44320.10561330422612877No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG42130.10039459627813187No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA42100.10032310712815931No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)