FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5606658
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2375314.236587999482044No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN795471.418795296592016No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT568971.0148113189711232No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA562501.003271467601555No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA536320.9565769840072286No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA518790.9253105860924636No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT475900.8488122514339203No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG470190.8386279312916892No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN422870.754228276452746No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA412430.7356075580140611No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT342860.6115229429011008No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC340970.6081519507699595No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN332130.5923849822835636No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA321170.5728367951103849No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA312730.557783264112061No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA311320.5552683969666065No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC221950.3958686261940714No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA219820.3920695715700868No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA212790.37953090771721765No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC197380.3520457284892355No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA192490.34332395519755265No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC189630.3382228771578363No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT188670.33651062718646296No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT160460.28619544834016986No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA140060.24981013644848676No Hit
AGGCAAGATGCTGGCATAGCTCGCCTTGGCCGTAC139580.24895401146280013No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN137030.24440584747633973No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCATAATG135450.24158776939845447No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT132400.23614780855190382No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA130750.23320487891360592No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA128650.22945933210122677No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT128550.22928097272920875No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA124610.2222536134716974No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA121600.21688499637395395No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA114780.20472088720232268No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC107540.19180766866821555No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA107070.1909693796197307No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG106700.19030944994326388No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA99890.1781631767088344No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA96740.17254485649026569No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC95420.17019051277962738No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAATG95340.17004782528201293No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC92180.1644116691262424No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT91190.16264591134326367No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA90790.16193247385519147No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT90530.16146873948794452No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC89980.1604877629418452No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT88460.1577767004871708No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA88080.15709893487350218No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT84720.15110605997369556No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGCGT84440.150606653732045No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA81350.14509534913668712No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA79660.142081075749582No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACGT79210.14127845857550078No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCGCGGCA78820.14058285702463036No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT78380.13979807578775094No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT78340.1397267320389437No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG73440.13098712281005906No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG72560.12941756033630014No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC70720.12613574789116797No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA69900.12467320104061994No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC66990.11948294331489455No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA66910.11934025581728011No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA66330.11830577145957538No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA64570.11516664651205763No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG62410.11131408407646765No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC61970.11052930283958823No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC61840.11029743565596475No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC58640.10458993575138702No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA58190.10378731857730576No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT58040.1035197795192787No Hit
CTGACCTATGAATTGACAGCCACGCCTTCCGGCAT57660.10284201390561008No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT56910.10150431861547468No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACTAATG56610.1009692404994205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)