FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263453
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC567062.5052872756801223No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT559732.4729031263295504No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA412691.8232762067513661No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA374531.6546842368717178No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA239741.0591781671631795No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT212440.9385659874536825No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA205610.9083908523835043No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA198490.8769344890306977No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA180710.7983819412198973No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA178780.7898551460975776No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA177090.7823886778298468No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT136170.6016029491224248No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG126920.5607361849351411No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC109720.48474609368959726No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA107600.47537987314072794No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG78260.34575491516722456No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC76190.3366095960463946No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC60900.26905793935195477No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT58140.2568641805241814No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC54210.2395013282802868No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA53960.23839682114008995No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA53250.23526002086193087No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT52130.23031182887384896No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51520.22761683145176861No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGCGT47870.2114910272048945No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC46950.20742644092897003No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT46360.20481980407810546No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43810.19355383124809747No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA42970.18984268725703604No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42700.18864981954562343No Hit
CTGACCTATGAATTGACAGCCCGCCTTAATTACGT42050.1857781009811116No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC40390.17844417357020445No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC39220.17327508015408316No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT39120.17283327729800443No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT38320.16929885444937448No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT37170.16421812160446891No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT36600.16169984532482007No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA35660.1575468984776799No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35010.15467517991316806No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT34910.1542333770570893No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33810.14937354564022315No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC32770.1447787959370042No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA30790.1360310993866451No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT29570.13064110454248443No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT28360.12529528998393163No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT27360.12087726142314419No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA27060.11955185285490798No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT26850.1186240668571426No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA26800.11840316542910323No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGATG26000.11486874258047329No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT25540.11283644944251106No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA25220.11142268030305909No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT25190.11129013944623546No Hit
CATTATTACTTTTGGTACGCGCGCCTTGCGGCATG25160.11115759858941184No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGAGT25120.11098087744698035No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG24980.1103623534484701No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG24670.108992764594626No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA24530.10837424059611576No Hit
TGACCTATGAATTGACAGCCACGCCTGTTAATGCA24480.10815333916807637No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23820.10523744031795668No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23640.10444219517701495No Hit
CATTATTACTTTTGGTACGCGACGCCTTGCGGCAT23550.10404457260654407No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA23460.10364695003607319No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC23330.10307260632317083No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT23280.10285170489513146No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA23110.10210064003979759No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA23080.10196809918297399No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA22940.10134957518446375No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG22890.10112867375642437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)