FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754698
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT692172.5126892312696345No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC664852.4135132054403057No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT450011.6336092014442236No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA268930.9762594665549545No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA256560.9313543626197863No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA236930.8600942825674538No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT223790.8123939538925864No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT202780.736124250280793No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA196960.7149967074430663No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT151580.550259955900792No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140870.5113809208849753No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG116840.42414812803436164No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC110320.4004794717969084No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT107970.39194859109782637No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT104820.3805135807990567No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA81810.29698355318804454No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC80540.29237324744854065No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA76800.2787964415700015No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT70440.2557086112524858No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70190.2548010707525834No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC66810.24253112319390363No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG66230.24042562923413022No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA62680.22753855413551685No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA61390.22285564515602072No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG54850.19911438567857528No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA52420.19029309201952446No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA51720.1877519786197979No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51310.18626361219995805No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT49370.1792210979207158No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA47270.17159775772153607No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA45650.16571689528216887No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA44460.1613970025026337No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA44310.16085247820269227No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA43540.15805725346299304No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGCATGT41850.15192227968365316No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA41200.14956267438390705No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGACGTG41170.14945376952391878No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC41050.14901815008396566No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG37860.13743793330521167No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA36720.13329954862565696No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT36630.13297283404569213No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA35300.12814471858621163No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC35190.1277454007662546No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT34860.1265474473063835No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA33700.1223364593868366No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA33580.12190083994688347No Hit
CATGCCTTGAGTGTAGGACGCAGTAAGGTTAATGC33330.12099329944698112No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATG32520.1180528682272975No Hit
CTGACCTATGAATTGACAGCTTATTCCATAGCATG32210.1169275180074186No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA32150.11670970828744204No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA31900.11580216778753968No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31050.11271653008787169No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA30770.11170008472798107No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT30460.11057473450810215No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT30390.1103206231681295No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC30380.1102843215481334No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC30280.10992130534817246No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT30130.10937678104823106No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA29660.10767060490841464No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG29610.10748909680843416No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA29140.10578292066861776No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA28960.10512949150868808No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC28680.10411304614879743No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG28000.10164453598906305No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA27770.10080959872915288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)