FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506902
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1158544.621401235469117No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA673672.6872610098041325No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT647932.5845844791699077No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA526522.1002815427168673No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC202370.80725134049915No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA195980.7817617122647793No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA194350.7752596631220526No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA186610.7443849021621108No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA179470.7159035335246452No Hit
GTCCAGTTTTCCCAGGAATCCCCCGCCTTGGCCGT172840.6894565483612841No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA172250.6871030459108494No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCCAGATTG166660.6648046074397802No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA123130.4911639944441386No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG114570.45701826397681283No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA112360.44820260225569253No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT100430.4006139849104592No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT94010.3750046870599648No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC92010.36702671265171116No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA77420.30882738934350046No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT73930.29490582400109777No Hit
TCCCTGAGACCCTTTAACCTGCCGCCTTGGCCGTA72450.28900212293899No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70020.2793088840329618No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC68920.2749209981084223No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT65810.26251524790358777No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC62040.24747676614402955No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT60430.24105449674538532No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA59370.23682617030901087No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG57460.22920720474912862No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA57300.22856896679646832No Hit
CAACGGAATCCCAAAAGCAGCCCGCCTTGGCCGTA55460.22122923034087494No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC52710.21025951552952607No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA47940.19123204656584103No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47450.18927744283581885No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG46740.1864452619208888No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC44860.17894596597713033No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC42860.17096799156887665No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41880.16705878410883235No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA41490.16550307909922285No Hit
ACTGGACTTGGAGTCAGAAGGCCGCCTTGGCCGTA41300.16474517153043877No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC41010.16358836524124198No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA40890.16310968677674675No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT40750.162551228568169No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG39530.15768466417913427No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA39450.1573655452028041No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38660.15421424531154387No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGCATGT35020.13969433188852218No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT32900.13123767901577324No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT31970.1275279209159353No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA31670.1263312247546972No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC31630.12617166526653215No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGCATGG31610.12609188552244963No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA31260.12469574000100524No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG31140.12421706153650999No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA28460.11352657582945007No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC27730.11061461517043747No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCACTCGGT26270.10479069385241227No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC26100.1041125660277107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)