FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816659
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAC394801.401660619904646No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA296051.0510679496524074No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT241490.8573632803970946No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC226910.8055998258930173No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT188950.670830228295296No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA178570.6339780569816935No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGTCTG154670.549125755016848No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT154050.5469245655934921No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG142770.5068771193105023No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA139450.495090104978984No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA131680.46750423107660527No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC122260.4340603530636829No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC119320.42362245483035044No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA106250.37721996166380095No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA101960.3619891509763873No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT99320.35261634439951733No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA88650.31473458448466785No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81190.2882493052939671No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA77300.27443861681517No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA73110.2595628366799105No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA70500.2502965392686868No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA68780.24419001377163513No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC66930.23762194855678304No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA65780.23353909720701016No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG65640.2330420544339943No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC62120.2205449789981677No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC61630.21880532929261226No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT61520.21841479568524272No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT58960.20932601355009606No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA55520.19711296255599275No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG54990.19523130062957567No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC53910.1913969706663107No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC52180.18525494211404364No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT49730.17655669358626658No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA49690.1764146813654049No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC48830.17336141861687906No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC48830.17336141861687906No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT48490.17215431473955492No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT47650.1691720581014599No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC46740.16594128007685702No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA46110.16370458759828577No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC45710.16228446538966912No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC45250.16065132484975994No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA44680.1586276507024812No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC44100.15656847349998704No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43470.1543317810214158No Hit
TGACCTATGAATTGACAGCCAGACGCCTGTTAGCA42860.15216609465327538No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT42630.1513495243833208No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA42220.14989389911948872No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG40170.14261577280032833No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA39770.14119565059171166No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT38690.1373613206284467No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA38570.1369352839658617No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC38250.13579918619896833No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC37980.13484060370815212No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG37610.1335269906651817No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT37480.1330654509473813No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA36720.13036721875100962No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGAGC35960.12766898655463796No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT35750.1269234223951142No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA35710.12678141017425254No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT34370.12202400077538672No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA34340.12191749160974047No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC33810.1200358296833234No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33630.1193967746894459No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33480.11886422886121464No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA32550.11556244472618091No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA32410.11506540195316507No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT31940.1133967583580405No Hit
CTGACCTATGAATTGACAGCCACGCCTTGTAATGC30730.10910088867697511No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG29790.10576360148672594No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT29410.1044144853885401No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA29150.10349140595293928No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28910.10263933262776928No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGTAATG28430.10093518597742929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)