FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997490
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1253276.274224151309895No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA697053.4896294850036798No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT588052.943944650536423No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN342621.7152526420657925No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA319571.5998578215660655No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN281981.4116716479181373No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT276271.383085772644669No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA254261.2728974863453635No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA240851.2057632328572359No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT210541.0540227986122583No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA200361.0030588388427475No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA195370.9780774872464943No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC161670.8093657540212967No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT141350.7076380857976761No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130890.6552723668203596No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA127380.6377003138939369No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124210.6218303971484213No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA123810.6198278839944129No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT123490.6182258734712063No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC123300.6172746797230524No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACCA116390.5826812649875595No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA110790.5546460808314435No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC101030.5057847598736415No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA100820.5047334404677871No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT97660.48891358655112166No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC94460.47289348131905545No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACTG93610.4686381408667878No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA88230.4417043389453764No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA77880.3898893110854122No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA76200.38147875583857743No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT73100.36595927889501323No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA72990.36540858777766094No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA69310.3469854667607848No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA59170.29622175830667485No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA57970.29021421884465004No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA56610.28340567412102186No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA55910.2799012761015074No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA52200.26132796659808055No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA51650.2585745110113192No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA50440.25251690872044413No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA49820.24941301333173133No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC48290.24175340051764965No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC47670.23864950512893685No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC47120.2358960495421754No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC45310.226834677520288No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGAGGT44260.2215780804910162No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA42600.21326765090188185No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA41270.20660929466480432No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGAGAC38970.19509484402925673No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC38440.19244151410019575No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA37570.18808604799022774No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT37480.18763548253057585No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT36420.18232882267245393No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC35900.17972555557224315No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT34250.171465188811959No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA34100.1707142463792059No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC33800.1692123615136997No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC33280.16660909441348892No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC31800.15919979574365828No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA31670.1585489789686056No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG30950.1549444552913907No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA30580.15309213062393304No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA29400.1471847168196086No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA29360.14698446550420777No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC29090.1456327691252522No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA28290.14162774281723564No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT28290.14162774281723564No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA28220.14127730301528418No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA28080.1405764234113813No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC27960.1399756694651788No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG26790.13411831848970457No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA26560.13296687342614982No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC25910.12971278955088636No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT25690.1286114073161818No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT25120.12575782607172No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC24530.12280411916955779No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC24080.12055129187129848No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT23920.11975028660969518No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC23850.11939984680774372No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG23810.1191995954923429No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT23520.11774777345568688No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC22780.11404312412077156No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT22730.11379280997652053No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC22670.1134924330034193No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA22290.11159004550711142No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG22190.11108941721860936No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC22000.1101382234704554No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG21670.10848615011839859No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA21370.10698426525289238No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC21240.10633344847783968No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA20460.10242854782752354No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT20240.10132716559281899No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA20010.10017572052926423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)