FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4987405
Sequences flagged as poor quality0
Sequence length35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1895743.8010548571852496No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1291852.5902247762112762No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN919341.843323331471978No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN638201.2796233712722347No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT576001.1549092163158998No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA555941.1146878988171203No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA497350.9972119769699874No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA467980.9383236372422131No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA451380.9050397952442202No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA411560.8251986754634925No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT407930.8179203413398349No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT402890.807814885697071No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT377100.7561046275568156No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC371560.7449966465526662No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN371480.7448362424948446No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT366590.735031544460496No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA298800.599109155963873No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT292470.5864171848887347No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA290040.5815449116324021No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT284660.5707577387438959No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN276970.5553388986857896No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC227510.45616908993755273No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT226190.45352242298349543No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAT195550.3920876688378024No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA189570.3800974655156339No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT183570.36806716117900995No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA183140.36720498936821855No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT176590.3540719071340707No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT168940.33873326910487517No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA161450.32371543919132295No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA149920.30059720435777726No Hit
CAAAGTGCTTACAGTGCAGGTCGCCTTGGCCGTAC145770.292276243858279No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAG144790.29031129414996376No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG126470.25357876490880527No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC125040.25071154237524323No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT122200.24501719832257457No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC119660.23992436948673707No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA118720.23803962180733268No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA109860.22027487240358465No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC109500.2195530541433872No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA105800.21213436646913575No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC104140.20880598226933644No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA102130.20477583031656743No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA99360.1992218398144927No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA97950.19639471829538604No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG96800.1940889099641998No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA95770.19202370771974606No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC91590.18364259569856467No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC90460.18137688838183386No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA88770.17798835266035143No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT84380.16918617998738822No Hit
CAAAGTGCTTACAGTGCAGGTAGTCGCCTTGGCCG80910.16222865397937405No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA79110.15861956267838687No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA77380.15515082492799362No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA76930.15424855210274682No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG75070.1505191577583934No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA72890.14614814718275335No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA72550.14546642993701134No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA71560.14348142972146838No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA70810.1419776416793904No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC70800.14195759117216267No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA70600.14155658102760854No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA68770.13788733820493823No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA65630.13159147893543838No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA65610.13155137792098295No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC64970.13026814545840973No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG63060.12643849857791778No Hit
CAAAGTGCTTACAGTGCAGGTATCGCCTTGGCCGT62190.12469410444910731No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT60820.12194718495891149No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA60820.12194718495891149No Hit
TAAAGTGCTTATAGTGCAGGTAGCGCCTTGGCCGT60780.12186698293000066No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT59820.11994213423614083No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA59580.11946092206267588No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG58630.11755612387604375No Hit
AATGACACGATCACTCCCGTTGACGCCTTGGCCGT58000.11629294192069824No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC55320.11091940598367286No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA53990.1082526885223879No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA53780.10783162787060606No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA51950.10416238504793575No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA50510.101275112007146No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC49960.10017233410962213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)