FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772170
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2288556.0669322962644845No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1086832.8811797983653973No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN623491.6528682429476933No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT601381.594254765824446No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN564741.4971223460236416No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA474041.2566771911128076No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT468731.2426004130248638No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA460041.219563275250055No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA339380.8996943403929304No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA332210.8806867134832206No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC327510.8682270417292964No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA282100.7478454046344677No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN267130.7081600246012242No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC256100.6789195608893555No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT246950.6546629658790564No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT221640.5875663079871798No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT221230.5864794004511992No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN179690.4763571100984314No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT167500.4440414933579346No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA167090.44295458582195396No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA155240.41154030703812394No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA146570.3885561891431192No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA141800.3759109478098813No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT128490.34062621780036423No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC127620.33831985302889317No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGAT127610.3382933430889912No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC119320.3163166029102612No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC118120.3131354101220252No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA117910.31257870138408395No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA117640.3118629330067309No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA115420.30597772634849435No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA113550.30102036758682665No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA105480.27962684608593996No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA102970.27297285117054637No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGCG93130.24688707030701162No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA92780.24595922241044277No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA89800.23805926031965685No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT86770.23002674852936109No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC86610.2296025894909296No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA85440.22650092652239956No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG84620.22432711145043832No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT83330.22090732920308467No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA79760.21144328065808277No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT78480.20805000835063106No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT77620.20577015351906197No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA74700.19802925106768782No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA73740.19548429683709906No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT72350.19179941519072577No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT69070.18310415490288084No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA68520.1816461082082727No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT68410.18135449886935107No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT66630.17663572956680107No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA66360.175919961189448No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC65350.1732424572593494No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTT64910.17207601990366286No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA61310.16253244153895502No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA56600.15004625984512893No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGCGT56350.14938351134757977No Hit
CGCCTTGGCCGTACAGCAGAAGTTTGCTGAGAGTC55850.14805801435248148No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC55010.1458311794007163No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC54560.14463823210512783No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGCGT54280.14389595378787276No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA54210.143710384208559No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC53790.1425969667326764No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA51650.13692383959365564No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA51510.1365527004350281No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA49720.13180742119257616No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG49290.1306674937767916No Hit
CGCCTTGGCCGTACAGCAGAAGTTTGCTGAGCTGA48480.12852018864473236No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA47750.12658496303188882No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACCTA47640.12629335369296718No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC47640.12629335369296718No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT46680.12374839946237841No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT43970.11656420574894556No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT43690.11582192743169052No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACCT43000.11399274157845485No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC41820.11086456867002283No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA40870.10834612437933602No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA39870.1056951303891394No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGGC39540.10482030237237451No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT38560.10222232826198183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)