FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779241
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2231475.904545383583635No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1504623.981275605339803No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1147043.035106784669197No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA621841.6454097529107032No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA581681.53914502938553No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA473391.2526060126887912No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC409701.0840801102655269No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA341920.9047319289772735No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT321950.851890630949442No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA318200.8419680036282416No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA205030.542516341244181No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA200270.5299212196311376No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT173440.4589281286903905No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG170660.45157215430294073No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA148140.39198346969669307No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA146200.38685016382919213No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC141350.3740168991604399No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT140400.3715031669057358No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAA139170.36824854514438216No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG127140.3364167566979719No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG116940.3094272103843073No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC116300.3077337486548225No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113790.30109220343449916No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT111360.2946623409303614No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC110010.29109019509472933No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC107040.28323147425633877No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT105840.2800562335135547No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA105210.27838923212359307No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACCTCACC101830.26944563736475124No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATCGT97980.25925840664831906No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT97200.25719450016550943No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC93070.2462663799424276No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAA91850.24303821852059712No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA89770.23753446789977142No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA88480.23412108410127852No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA88190.2333537342551057No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA87910.2326128447484561No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC87740.23216301897656172No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA87630.23187195524180648No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC86890.22991389011708965No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC80900.21406414674269253No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA79040.2091425235913772No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAGCA78660.2081370306894956No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA76210.20165424750631145No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC74340.19670616401547295No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC73380.1941659714212457No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT73090.1933986215750729No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC69630.18424334410004548No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68740.18188837388248064No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG66630.1763052422430853No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGATG66560.1761200198664229No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC65250.17265371538888363No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65220.17257433437031405No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA61460.16262524670959064No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT61140.1617785158448482No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA60220.15934416460871376No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC59010.15614246352640648No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT56480.14944799762703675No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC55890.14788683759516794No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT55520.1469078050328095No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53630.1419068008629246No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT52980.14018687879391656No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT52680.13939306860822054No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA52460.13881094113871012No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC52230.1382023533296765No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG51210.13550339869831005No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG50890.13465666783356764No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT49230.13026425147271636No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC49220.13023779113319314No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA48140.1273800744646875No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA48000.12700962971136268No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA47140.12473404051236744No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA46940.12420483372190341No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC45890.12142649807196737No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA44590.11798665393395129No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG44320.11727222476682488No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA43590.11534061998163124No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAACAT42720.11303857044311279No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA42460.11235060161550957No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC42280.11187431550409195No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT42150.11153033109029034No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT40400.10689977167373024No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG39610.10480940485139742No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG39610.10480940485139742No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT38670.10232213293621656No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC38280.10129017969481174No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38060.10070805222530133No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA37920.10033760747197651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)