FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008440
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2584148.589634494954195No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1526045.072529284280225No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA434321.443671803326641No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT419271.393645876268099No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC395291.3139367911608675No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA387431.2878102937070375No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT330921.0999720785523395No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT260260.8650995200170187No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA259520.8626397734373962No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA257640.8563906875324089No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA236590.7864208692877371No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC202230.6722088524285011No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT173120.5754477403571285No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGT161620.5372219489170468No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT118110.39259549799896293No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC111660.3711558149738735No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA107790.3582920051588198No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA106390.3536384305487229No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA106380.35360519073007934No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC105610.35104572469452605No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA102620.34110701892010475No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA98610.32777785164404144No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT89150.29633298320724366No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86930.2889537434683756No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC84070.2794471553363205No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA83310.27692092911941074No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA83210.27658853093297525No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA83200.2765552911143317No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA81080.2695084495618992No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA78260.26013482070441823No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT77500.2576085944875085No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA77100.2562790017417665No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT74800.24863384345375014No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT70660.23487255853532063No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC66680.22164311071518794No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA65620.2181196899389717No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGCTTG65560.21792025102711038No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC63210.21010889364587626No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC60660.20163273989177113No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA60350.20060230551382113No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC58750.19528393453085321No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT55460.18434803419712542No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGG55260.18368323782425444No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT54920.18255308399037373No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA54110.17986065868024625No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC52810.1755394822565848No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG52060.1730464958583186No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA51490.1711518261956363No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGAC50380.16746220632620226No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA50250.16703008868383615No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA48890.16250947334831342No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA48520.1612796000585021No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46700.15522995306537607No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC45770.15213864993152598No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG45720.15197245083830824No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC43790.14555716584010317No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT43380.14419433327571765No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG42670.14183430615202564No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT41450.13777904827751258No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40180.13355759130978181No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT39880.13256039675047532No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA39440.13109784473015915No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC38580.1282392203268139No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA37150.12348592626078632No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35740.11879911183204585No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC35330.11743627926766032No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG33250.11052239698980201No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA33230.11045591735251493No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG33070.10992408025421814No Hit
CTGACCTATGAATTGACAGCCGCCTTGTAATGCAC33010.10972464134235685No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA32150.10686601693901158No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC31660.10523726582547767No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC31360.10424007126617117No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA30920.102777519245855No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC30430.10114876813232107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)