FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905084
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2096015.367387743771965No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA826942.117598494680268No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT423041.0833057624368643No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT385360.9868161606766974No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA378930.9703504457266476No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA367620.94138820061233No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAC288550.7389085612498988No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT277530.7106889378051792No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC270500.6926867642283752No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA218650.5599111312330285No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA218000.5582466343873781No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC198910.5093616424128137No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA192540.49304957332543936No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC156160.39988896525657325No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC151460.3878533726803316No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA141770.3630395658582504No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA139190.35643279376320713No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA134720.344986176993888No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC130780.33489676534486834No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT127970.32770101744290264No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC116890.29932774813550744No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT116290.2977912895087532No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA107660.27569189292727125No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT102260.261863765286483No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97930.2507756555300731No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCGT97240.2490087281093057No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT95110.24355429998432812No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC90270.23116020039517715No Hit
TAGCTTATCAGACTGATGTTCTGGCGAGGTTAATG89230.22849700544213644No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA87860.22498875824438092No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA85930.22004648299498805No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA83340.21341410325616555No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA82590.21149352997272275No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA82520.21131427646626805No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA79980.20480993494634173No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAG72160.184784757510978No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC69240.17730732552744063No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT68310.17492581465597154No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67550.1729796337287495No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT67490.17282598786607406No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA67340.1724418732093855No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA67050.17169925153978763No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA66670.17072616107617658No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA64730.1657582781830045No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA63910.16365845139310703No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT63500.1626085379981583No Hit
TACCCTGTAGATCCGATAAAGTCGCTCCAAGGCAT63150.161712270465885No Hit
TAGCTTATCAGACTGATGTTCTGGCGCTTGGCCGT62540.1601502041953515No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA61310.15700046401050527No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA59280.15180211232332005No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA57960.1484219033444607No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT54760.1402274573351047No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA54650.13994577325353308No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT53920.13807641525764874No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGGT52220.13372311581517837No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT51860.13280124063912582No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA51430.1317001119566186No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG51380.13157207373772242No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA51140.13095749028702072No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA50260.12870401763444783No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC49710.12729559722658976No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT49570.1269370902136804No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG47400.12138023151358587No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG46010.11782076902827188No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA45660.11692450149599855No Hit
TAGCTTATCAGACTGATGTTCTGGCGACCAATTAC44800.11472224413098411No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA44610.1142356988991786No Hit
CCACTACGCCTTGGCCGTACAGCAGCGTGTCGCAC42940.10995922238804595No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG42740.10944706951246119No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA42350.10844837140507092No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC42240.10816668732349932No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC41940.10739845801012218No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC41010.1050169471386531No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC40890.10470965541330224No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC40700.10422311018149673No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA40640.10406946431882132No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC40460.10360852673079503No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG40440.10355731144323656No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA40360.10335245029300266No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT40020.10248179040450858No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC39500.10115019292798824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)