FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5640182
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1153472.045093580313543No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1077631.9106298342854895No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC611191.0836352443945958No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN573091.016084232742844No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA559690.9923261341566637No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT505940.8970277909471716No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA471510.8359836615201424No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN444460.7880242162398305No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA407650.7227603648251067No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA387370.6868040783081114No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT377170.6687195555037053No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN357490.6338270644457927No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA351510.6232245697036017No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC341520.6055123753098748No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC320570.5683681838635704No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA292520.518635746151454No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC271320.4810483065971985No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT263420.46704166638594286No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC255210.4524853985208278No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT252900.44838978600335944No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC244590.433656218895064No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA242640.4301988836530452No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT228970.4059620771102776No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC228380.404916011575513No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA228120.4044550335432438No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA224560.3981431804860198No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA191760.339989028722832No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA190150.33713451090762675No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA188760.33467005142741846No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA179890.3189436085573125No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA178970.3173124555200524No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC177610.31490118581279825No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA162210.28759710236300884No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT158780.2815157383219194No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA148390.2630943469554706No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA148380.26307661703115254No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA147710.2618887121018435No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA139580.24747428363127288No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA139060.24655232756673456No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA131490.23313077485797445No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT129590.2297620892375459No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA128120.22715579036279324No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG121090.21469165356720757No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT118930.21086198991450986No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT117340.20804293194794068No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC115660.20506430466250913No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC111930.1984510428918783No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA107320.19027754778125955No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC104980.186128745490837No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA100370.17795525038021823No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA99570.17653685643477462No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA97320.17254762346321448No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC96530.1711469594420889No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT95400.16914347799414983No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC94160.16694496737871223No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTA93730.16618258063303631No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA93590.1659343616925837No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA92240.16354082190964758No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG87560.1552432173288025No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC87310.15479996922085137No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA86730.15377163361040477No Hit
TCAGTGCACTACAGAACTTTCGCCTTGGCCGTACA86480.15332838550245365No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT84420.14967602109293635No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC84170.14923277298498522No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG83930.14880725480135215No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC82990.14714064191545592No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA82530.14632506539682583No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT82520.1463073354725078No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA80930.14348827750593865No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG80630.1429563797763973No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT79280.1405628399934612No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTT78590.1393394752155161No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT77510.13742464338916724No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA77350.13714096460007852No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC76320.13531478239531988No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAAGG74840.1326907535962492No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC74280.13169787783443868No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC72370.1283114622896921No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA72350.12827600244105597No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC71280.12637890053902515No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA69850.12384352136154471No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG69020.12237193764314698No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA68370.12121949256247405No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG65830.11671609178569062No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA64220.11386157397048535No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA63250.11214177131163498No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG63150.11196447206845452No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG63030.11175171297663798No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA60820.10783339970235004No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA58730.10412784551987861No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAGAA58000.10283356104466132No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC57740.10237258301239215No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCGAT57580.10208890422330344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)