FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487056
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1128074.535764373620859No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA679742.7331109552820685No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT559062.247878616323878No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN523782.106024150642366No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA478601.9243635848971634No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA360261.4485399604994822No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA350681.410020522256033No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT312401.2561036020097658No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN274021.1017845999446736No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA255381.0268365489156657No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT252081.0135678488944357No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA238690.9597290933537483No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA229330.9220942351117143No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA196600.7904928558102431No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA193180.776741657606423No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN191910.7716352185073436No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA190060.7641967048590783No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA171610.6900126092858384No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA141100.5673374463622853No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC139630.5614268436255556No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA133890.5383473472249921No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA128390.5162328471896089No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT122520.4926306444245727No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT120880.48603650259584025No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT118550.47666799621721423No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC101250.4071078415604635No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT96650.3886120778945066No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC94770.3810529397005938No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA91890.3694729833184295No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87340.3511782605618852No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT83760.3367837314479449No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA82450.3315164596213354No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC82270.33079271234745017No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT79620.32013754414858375No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT75260.3026067768478072No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA74990.3015211559369793No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA71810.28873495409833955No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAACAT67320.27068148043309037No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC66910.26903294497590724No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT65170.26203672132834965No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT61990.24925051948970992No Hit
AGGCAAGATGCTGGCATAGCTCGCCTTGGCCGTAC60320.24253575311532993No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT58210.23405182673811928No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT56240.22613081490726383No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA56000.2251658185420835No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT55010.22118520853571452No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA54780.22026042035241666No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA54220.21800876216699583No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA54200.2179283458032308No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA53430.21483231579827716No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC53360.21455085852509956No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA51700.20787630033260207No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT50660.20369464941682056No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT50100.2014429912313997No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC47550.1911899048513584No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC45810.1841936812038008No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA45700.18375139120309314No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC45110.18137910847202474No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC44430.17864495210401374No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT43780.17603142028165028No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA43560.17514684028023494No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA40550.16304417753359796No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC38910.15645003570486551No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA38470.15468087570203484No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC38130.15331379751802934No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC37940.15254984206226158No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA36950.1485692320558926No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA36270.14583507568788157No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG35430.14245758840975034No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA34850.14012551386056446No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG32880.13220450202970901No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC32680.13140033839205872No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA31630.12717847929439466No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA31570.12693723020309958No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA30950.12444432292638365No Hit
TATTGCACTTGTCCCGGCCTGTTCGCCTTGGCCGT29540.11877496928094905No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA29250.11760893200635611No Hit
TATTGCACTTGTCCCGGCCTGACGCCTTGGCCGTA28920.11628206200423312No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC28720.11547789836658283No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA28430.11431186109198989No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC27850.11197978654280402No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA27600.11097458199574115No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA27560.1108137492682111No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC27060.10880334017408534No Hit
TCGAGGAGCTCACAGTCTAGTCGCCTTGGCCGTAC26510.10659189017054702No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA26330.10586814289666176No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC25990.10450106471265624No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC25820.10381752562065351No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT25790.10369690107500595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)