FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229228
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT25302211.350207336351419No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1109674.977821918619361No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC448362.01127924106462No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC419021.8796641707353396No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN347041.5567721202138138No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN327761.4702847802019354No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT290651.303814594110607No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA265541.1911747026324808No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT223521.0026789543285837No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA195330.8762226205664023No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG190740.8556325328768525No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA176020.7896007048179908No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN160710.7209222206073134No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA158320.7102010202635173No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA135870.6094935107579844No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133610.5993554719391646No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA132330.5936135738470897No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA126570.5677750324327525No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA100140.44921380854717424No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA100120.4491240913894855No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT99180.444907384978118No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA99160.4448176678204293No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGAGAG90960.40803363316807434No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA86950.39004534305149585No Hit
GTCCAGTTTTCCCATTCCGAAAATAAGGTTAATGC85820.38497632364208595No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC84980.3812082030191618No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA73910.33154975623848254No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT67630.30337856872423996No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG67510.30284026577810796No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC67460.30261597288388625No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA58510.2624675448182061No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG57840.2594620200356357No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT57420.2575779597241735No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA55020.2468119008015331No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA54800.24582501206695773No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA54760.24564557775158036No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT54540.244658689017005No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT54310.2436269417035853No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC53480.23990367965950546No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA52280.23452065019818522No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT51670.23178427688868075No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT51160.22949648936761965No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG47290.21213621935486185No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA46660.20931012888766873No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC44660.20033841311880166No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA43170.1936544848709957No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA43030.193026464767175No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA42580.19100782871917993No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG42030.18854060688274146No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC41790.18746400099047741No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA41700.1870602737808784No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA41600.18661168799243505No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC39560.17746053790819064No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT38800.17405128591602115No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC35650.15992083358005552No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC34770.155973278641754No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC34090.1529228952803392No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC31940.1432783008288071No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA31040.13924102873281693No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT30730.13785041278864252No Hit
GTCCAGTTTTCCCATTCCGAAAGATAAGGTTAATG30420.13645979684446813No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC29000.1300898786485725No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT28260.1267703438140917No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT27680.12416854624112024No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC27540.12354052613729954No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA27280.12237420308734684No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC27080.12147703151046013No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT26670.11963782977784237No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA26270.11784348662406896No Hit
GTCCAGTTTTCCCAGCTTAGGGAGCGGAACCGGCA26250.11775376946638028No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGAGAA26150.11730518367793694No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT24860.11151842700701767No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA24540.11008295248399894No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA24510.10994837674746594No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT24430.10958950811671125No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA24300.1090063465917349No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA24160.1083783264879142No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA23060.1034438828150373No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG23040.10335416565734863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)