FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666535
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2677204.724580365249663No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1337292.3599783642031684No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1187612.0958310501920483No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT1002181.768594034979048No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA683571.2063280293865652No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA635361.1212495819755812No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN587251.0363476092532737No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA569071.00426451085187No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT549460.9696578244023906No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA548980.968810745896743No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA457320.8070540462557807No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT442160.7803004834524097No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA356350.6288675530990279No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT348220.6145201609096211No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA347810.6137966146860471No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN321940.5681426127254133No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA321210.5668543474980742No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT303420.5354595003825089No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA278390.4912878858067585No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC262050.46245192167700366No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA251060.4430573533914465No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT238930.42165097365497606No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT236970.418192069756915No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT233300.4117154486824841No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA226660.39999752935435856No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN224310.3958503741704587No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT194090.3425197232523932No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGTG191980.33879610732131715No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA190170.3356019154562709No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT180100.3178309143065383No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC174240.30748949755009014No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT174140.30731302286141354No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA171200.3021246670143218No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT163700.28888906536357756No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA159500.28147712843916084No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC143050.25244704215186176No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA138110.2437291925312382No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC133470.23554076697664444No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT133190.23504663784835No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT129340.2282523623343013No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA128530.22682291735602092No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA127540.22507581793812267No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA125580.2216169140400615No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT124860.22034629628159005No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG115860.20446357430069698No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA109280.19285153978577738No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA108460.19140444733862935No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA105100.18547489779909593No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA104420.1842748699160951No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG97770.1725393031191019No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA97390.17186869930213086No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA96960.1711098581408215No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT91810.16202141167397713No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC91020.16062726163343208No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA89950.15873898246459256No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG88690.15651540138726755No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC88350.1559153874457671No Hit
ATCACATTGCCAGGGATTTCCATCGCCTTGGCCGT87840.1550153665335165No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA85740.15130939807130814No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT81110.14313861998558203No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTGT79910.14102092372146294No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGT79220.13980324836959446No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT78700.1388855799884762No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATTAC78550.13862086795546133No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG76270.13459724505363507No Hit
TAGCTTATCAGACTGATGTTGACGCGGTTGGCCGT75600.13341486463950192No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG75480.13320309501309No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA75220.13274426082253088No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT74780.13196777219235387No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC74240.1310148088735003No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC73780.130203025305588No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA70880.12508525933396689No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC70080.12367346182455416No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA68830.12146752821609678No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT68240.12042632755290492No Hit
TAGCTTATCAGACTGATGTTGAACGCCTTGGCCGT65580.1157321008341076No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC59710.1053730366087918No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA59690.10533774167105647No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC59500.10500243976257095No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG58290.10286709602958422No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG57960.10228472955695148No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA57720.10186119030412766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)