FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766891
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC710162.5666352595747357No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT485981.7564117993806045No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT366231.3236155670751033No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN351101.2689332539662748No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN298151.0775632289092703No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA295301.0672628592886384No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA279061.0085688232749321No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA272450.9846791940846242No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT255250.9225155598829156No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC238580.8622674330141664No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC191560.6923294050976349No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC152680.5518106784835398No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA151890.5489554883079962No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT138550.5007425301538803No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA137380.4965139573622524No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128970.46611883156944023No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA122590.4430604602783413No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120670.43612126390233663No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA113960.4118702182341119No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG109850.3970160009917268No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA103600.3744274711219199No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT102650.3709940145817092No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT98940.3575854632509918No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC94360.3410325885623973No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT89660.3240460141003024No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC89210.3224196399496764No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA86170.31143257902100224No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA86020.31089045430412693No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC85360.30850510554987526No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT85340.3084328222542919No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC73570.2658941028034715No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT69580.25147358533458675No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT67520.24402840588949837No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA65710.2374867676392023No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC64900.2345592941680753No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA62820.22704183142740353No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA62180.2247287659687353No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA61590.22259640874902556No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA61200.22118688448514956No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA59620.21547650413406239No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC55530.20069457018726072No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC55110.19917662098000968No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT54250.1960684392699243No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG53320.19270726602529697No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT53310.19267112437750528No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT50880.18388870396412435No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC50160.1812865053231226No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT49860.18020225588937186No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG46720.16885377848278085No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC46670.1686730702438224No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT46400.16769724575344674No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA44140.15952923335252456No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA42590.15392727794481242No Hit
CTGACCTATGAATTGACAGCTATTCCAATTTGCAC41570.15024082987005993No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC41210.14893973054955906No Hit
CTGACCTATGAATTGACAGCTATTCCAATTCATGT40950.14800004770697509No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC40020.14463887446234783No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG39400.14239809229926295No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATA39160.14153069275226238No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC38750.14004888519280304No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC38410.13882006916788556No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA37910.13701298677830098No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA37120.1341577966027574No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA35060.12671261715766902No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGC34930.12624277573637704No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT34930.12624277573637704No Hit
CTGACCTATGAATTGACAGCTGTATCTTGCTTACG34750.12559222607612658No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC34530.12479710982470939No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC34240.12374900203875035No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC34080.12317073567408329No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC33430.12082152856762336No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC32810.11858074640453853No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA32490.11742421367520442No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG32010.11568941458120323No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC31300.11312335758799316No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC30980.11196682485865904No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCG30170.10903935138753208No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC30120.10885864314857362No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAG30080.10871407655740685No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC29950.10824423513611486No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG29800.1077021104192395No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG29750.10752140218028106No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC28710.10376267080994517No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA28410.10267842137619443No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA28300.10228086325048584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)