FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585649
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN471131.3139322895241559No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC276610.771436356430872No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN241930.6747174639793243No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN236910.6607172090742847No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211530.5899350438372524No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG189320.5279936770163505No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG135560.3780626603440549No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC112400.31347184289371327No Hit
TCCCCGGCATCTCCACCACGCCTTGCGGCATGTCG107560.2999735891605676No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC101820.28396532956795273No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC77150.21516328006450156No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGTCGT72410.20194391587129695No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGCGG68970.19235011569732563No Hit
TCCCCGGCACCTCCACCACGCCTTGCGGCATGTCG66310.18493165393489436No Hit
TCCCCGGCATCTCCACCACGCCTTGCGATGCACTA65890.18376031786714206No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG58010.1617838221197892No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGCGG55240.15405858186342278No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATGATG55140.15377969232348174No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC52370.14605445206711534No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAT49260.13738098737494941No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTTCGTA47510.13250042042598145No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATCGTAC47290.1318868634381112No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA44660.12455206853766222No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG43650.12173528418425786No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGCCTAC43160.12036872543854683No Hit
TCCCCGGCACCTCCACCACGCCTTGCGATGCACTA42750.11922527832478863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCG42600.11880694401487708No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC39430.10996614559874655No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGCG38930.10857169789904143No Hit
TAGCACCATCTGAAATCGGTTACGCCTGCGGATGC37110.10349590827211476No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC36670.10226879429637424No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGCCTACG36090.1006512349647163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)