FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071219
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2152527.008682871524304No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1034793.369313617817551No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT591701.9265965728917411No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN558851.8196357863115589No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN432461.4081053809578543No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT429851.3996071266816206No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT394701.2851574570227653No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA389071.2668259736606216No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA385481.2551368039856485No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA339171.104349771214622No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA313061.019334668091074No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT288820.9404083525140994No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA280230.9124390022333152No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA241860.7875048962643173No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA240580.7833371700292295No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT236790.7709967931300242No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC216070.7035317246995412No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA210770.6862747332573809No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205040.6676176462831208No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA196920.6411786329792829No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA186420.6069902537070785No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147730.48101421617930856No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT139590.4545100821530474No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC138970.45249133975792677No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA131220.4272570598189188No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA126690.4125072161900535No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA121130.39440365535639105No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA117130.38137951087174177No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAC114800.3737929467094336No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC114360.3723602908161222No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA113470.3694624186682878No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT110350.3593035859702613No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA101940.3319203221912863No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA98930.3221196534665877No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAG95800.31192826040734967No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT92650.30167174662568835No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC87910.286238135411379No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA85890.27966094244663114No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA83350.2713906106988788No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT82780.26953467010981635No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA79930.26025496716450375No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC75490.24579816678654307No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT73740.24010010357450903No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT71320.2322204961612962No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA66080.21515886688640568No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC64390.20965616584164137No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA61430.2000182989230009No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG60870.19819491869515005No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA59680.19432023571096688No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT57370.18679879227108195No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA56180.18292410928689878No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT52510.1709744567222331No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT52500.17094189636102147No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA52330.17038837022042388No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG50420.16416934122900384No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT49210.16022953752239746No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC49000.15954576993695335No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC48880.1591550456024139No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC48810.15892712307393253No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA48210.15697350140123514No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA47930.1560618112873097No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA47460.1545314743103634No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC46520.15147080035647084No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA45020.14658674617472736No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC44850.14603322003412977No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG44040.1433958307759883No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC43830.14271206319054422No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA40100.13056704845860878No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA39810.1296227979834717No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA38750.12617139969503965No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC37510.12213391490479839No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA37120.1208640608175451No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC36830.119919810342408No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC36550.11900812022848256No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA35350.11510087688308779No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35150.11444966965885532No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA34690.11295189304312066No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC34330.11177972003950222No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA33480.10901208933651427No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC33260.10829576138985855No Hit
CTCCTGACTCCAGGTCCTGTGTCGCCTTGGCCGTA33170.10800271813895396No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA32570.10604909646625656No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA32200.1048443631014265No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAA31540.10269537926145937No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC31360.10210929275965015No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA31260.10178368914753394No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT30870.10051383506028061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)