FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200821
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1305084.077328910301451No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA938302.931435403604263No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT557541.7418656026063313No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA548881.7148100440480738No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA510751.5956843572320978No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT464811.4521586805385245No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN454471.4198544685879029No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA406541.2701116369831364No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT364691.1393639319412114No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN293340.9164523726881322No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA286130.8939269018792366No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC229510.7170347857627778No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA221310.6914163584905247No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA206660.6456468512297314No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA195930.612124201884454No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178000.5561073237147595No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT177230.5537016909099259No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA156300.4883122173967242No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA148010.46241261226416597No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA136820.4274528316328842No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTCAAA129010.40305284175528716No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC122100.3814646304807423No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT118740.37096732369601426No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA115580.36109485660085333No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT114380.3573458184634505No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112110.35025388798686335No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT109460.3419747621000987No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA107550.33600754306473246No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA102220.31935556533776804No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA97550.30476555858637516No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC97440.3044218967571133No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC89910.28089668244491023No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTGTTT88880.27767875804363945No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC88590.2767727404937671No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC85500.2671189672899547No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC82330.25721525821031543No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80610.25184163688003797No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA80450.25134176512838424No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA79230.24753024302202464No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA73100.22837890653679163No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT69580.21738172800040992No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT69010.2156009348851435No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT67810.21185189674774066No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT66210.20685317923120347No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC65660.20513487008489384No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA63720.19907392509609254No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG62270.19454383734673072No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC60460.18888903815614805No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA59930.1872332129787951No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC58570.18298430308973854No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC57590.17992258861085952No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT56760.17732950389915586No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA56320.17595485658210816No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG56160.17545498483045444No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC50820.15877176511901167No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT50170.15674103612791843No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG50110.1565535842210483No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC50010.15624116437626473No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA49800.1555850827022192No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTCAAC49060.15327317585082076No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48950.15292951402155885No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47940.14977407358924477No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTGTTG47750.14918047588415598No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT47670.14893054000832912No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA47590.14868060413250225No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA47250.1476183766602381No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46240.14446293622792403No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA46150.14418175836761882No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC45840.14321325684878974No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA45230.14130749579560994No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA44500.13902683092868987No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC42890.13399687142767433No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA41180.12865449208187524No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT39050.12199994938798515No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG37490.11712619980936141No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC36330.11350212960987197No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA35460.11078407696025488No Hit
CGCCTTGGCCGTACAGCAGCGCAGGTTAATGCACT35050.10950315559664224No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCC34730.1085034120933348No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA33510.10469188998697522No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA33370.1042545022042782No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT32700.10216128924422826No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT32140.10041173811344029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)