FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3476046
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2016395.800815064012387No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1317123.7891328250546743No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT880102.531899750463601No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA601131.7293499568187531No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT598731.7224455602716418No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA550931.5849329957083422No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA468681.348313572375049No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA392391.128840067133749No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA259080.7453296072606634No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC242790.6984660156971455No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT205990.5925986019747724No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT204610.5886285739601835No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT174580.5022373121644534No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA169010.4862133585113661No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA163530.4704483197287953No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC156730.45088586284531335No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA155400.44705967642545585No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA144950.4169967831265754No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT124050.35687099652881465No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC117100.3368770148611382No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA110020.3165090450471599No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA108390.3118198090589135No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA102120.29378207307958526No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGAT99580.2864749200672258No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT97640.2808938661916442No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT91950.26452469271120116No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT90700.26092865284291406No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT89580.25770660112092875No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC87520.2517803274179916No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA85090.2447896259140414No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA83760.24096343949418392No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82710.23794276600482273No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA79600.22899581881252437No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG76040.21875429726764262No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC73890.21256910869418877No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA73240.21069916796267943No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT72180.20764972615437194No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA71490.20566471214707743No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC71170.20474412594079597No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC69060.19867401064312729No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG68850.19806987594525502No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT67880.19527934900746424No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT67530.19427245784434383No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC67510.19421492120645123No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA64300.1849802908246899No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT61150.17591827035660632No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT56830.16349035657180602No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA54260.15609689860260767No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA53130.1528460785616761No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCAATTAC52200.15017062489967048No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA51910.14933634365022788No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA50960.14660335335032965No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50340.14481971757565926No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCCAT49760.143151155076774No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA46770.1345494277118312No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT46430.13357130486765711No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAT46030.13242057210980523No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT45090.1297163501288533No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44990.12942866693939034No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA44990.12942866693939034No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGCG44840.12899714215519587No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG43240.12439421112378836No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC42530.12235166047860126No Hit
ACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGTA42350.12183383073756791No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG41700.1199638900060586No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC40540.11662676500828816No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG40220.11570617880200665No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC39790.11446914108731589No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC39250.11291565186421584No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT38470.11067172298640468No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37790.10871547729805646No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA37770.10865794066016388No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAA36750.1057235721276416No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC36480.10494682751609155No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA36480.10494682751609155No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACC36320.10448653441295082No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA36030.10365225316350819No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA35780.10293304518985077No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA35700.10270289863828039No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC35520.10218506889724703No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT35190.10123571437201924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)