FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5295500
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2100923.9673685204418843No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1399292.642413369842319No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1119362.1137947313756964No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN898341.6964214899442924No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT697481.3171183079973563No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN640271.209083183835332No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA598831.1308280615617035No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT586901.108299499575111No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA538771.0174110093475592No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA533141.006779340949863No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA524230.9899537343027098No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA440040.8309696912472855No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA423690.800094419790388No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN384810.7266735907846285No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC381940.7212538948163535No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN379140.7159663865546219No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA351870.664469832876971No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT292250.5518836748182419No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC271420.5125483901425739No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA247400.4671891228401473No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT225640.42609763006326123No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA217460.4106505523557738No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC188630.3562081012180153No Hit
CCACTACGCCTTGGCCGTACAGCAGATACCGACCC172590.32591823246152396No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC144990.2737985081673119No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT142050.26824662449249365No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA140200.2647530922481352No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC137350.2593711641960155No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC136350.2574827683882542No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC135390.2556699084128033No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA134920.25478236238315555No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA129920.245340383344349No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG128990.24358417524313097No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA125480.23695590595788876No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA118820.2243791898781985No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG117670.22220753469927296No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT114090.21544707770748747No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA112420.21229345670852612No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGAAAC106360.2008497781134926No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA106200.20054763478425078No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG106090.20033991124539705No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT104390.19712963837220282No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC103770.1959588329713908No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGACCA102680.19390048154093098No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA102130.19286186384666226No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT92380.17445000472098954No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT91860.17346803890095364No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT90250.17042772165045794No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA88610.1673307525257294No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA88350.16683976961571145No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT87880.16595222358606365No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA87760.16572561608913228No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA87580.16538570484373524No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG87080.1644415069398546No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC86760.163837220281371No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC83820.15828533660655272No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA82270.15535832310452272No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC81620.15413086582947785No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC81500.1539042583325465No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA81120.1531866679255972No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT80590.15218581814748372No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT80010.15109054857898213No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA79230.14961759984892833No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG77520.14638844301765652No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC76890.14519875365876686No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA75430.14244169577943538No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA74810.14127089037862334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC72030.1360211500330469No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG71710.1354168633745633No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC69270.1308091776036257No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA67530.12752336889812105No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC67380.12724010952695686No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG65280.1232744783306581No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA63930.12072514399018035No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA63410.11974317817014446No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA60090.11347370408837693No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC59470.11230289868756492No Hit
CCACTACGCCTCCGCCTTGGCCGTACAGCAGATAC58350.11018789538287226No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA58060.10964026059862148No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA56070.10588235294117646No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT55890.10554244169577943No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA55230.10429610046265697No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA54940.1037484656784062No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT54810.10350297422339723No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC53770.10153904258332547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)