FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002406298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9562951
Sequences flagged as poor quality0
Sequence length35
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT5464965.714721323992981No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3268563.417940759081585No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1520261.5897394015717534No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1303671.3632507371417044No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA1186441.2406630547411568No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1175001.2287002202562787No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN841240.8796866155645887No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT773270.8086102292064447No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA707710.7400539854277199No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA689070.7205620942740374No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA649410.6790895404567063No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN642610.6719787647139466No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT605050.6327021857583501No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT555260.5806366674889372No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT545130.5700437030368555No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA528540.5526955016291519No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA504490.5275463609507149No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA496020.5186892623417186No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC414810.4337677773314953No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG370400.3873281375173835No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA348500.3644272568164367No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG295050.30853446807371493No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC291740.30507319341069505No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT268490.2807606145843474No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT244580.2557578722300261No Hit
GTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGCA236170.24696351575993644No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT231440.242017343809458No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA228180.23860835426219376No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT222790.23297201878374152No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA217210.2271369998654181No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA216130.22600764136509743No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA209180.21874001027507092No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT207350.2168263750384165No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA201050.21023845045321263No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA196740.20573147347508108No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC194720.20361915479855539No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA186840.19537902055547499No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC185460.1939359513606208No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA176970.18505793870532222No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA171170.17899286527767422No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA170480.17827133068024711No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA156860.1640288651484254No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC154030.16106952759665924No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA149810.15665666382688775No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA148470.15525542272463805No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC147950.15471165752077995No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC145350.15199283150148943No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA138790.1451330243143565No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA136600.14284293624426184No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA135520.1417135777439412No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT134990.14115935551693196No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA131570.13758305359924985No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT121210.12674957761469235No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG118590.12400983754909964No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC116210.12152106603913373No Hit
CGCCTTGGCCGTACAGCAGAGCTTACCGCAGATAC115790.1210818710667868No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA115210.12047536372402201No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA114190.11940874736260805No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC113280.11845715825585638No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC112960.11812253351502064No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA112940.11810161946871839No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA112920.11808070542241615No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC109470.1144730324352807No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG108950.1139292672314226No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA108870.11384561104621366No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA103550.10828247472981928No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC102570.10725768646100979No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT101720.10636883949316484No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA101640.10628518330795587No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG97100.10153769479734866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)