FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054308
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT744842.438653862020464No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC621332.034274212031007No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA417141.365743075027142No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA397741.3022262325868905No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT312341.0226211632880509No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT301510.9871630496989825No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC270040.8841282542559559No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA269060.88091967149351No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA258730.8470985899260979No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT211230.6915805478687808No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT204270.6687930621273297No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA201850.6608698271425147No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA185580.6076008051578295No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG175650.5750893492077419No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA149880.4907167188115933No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC137290.449496252506296No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA134370.43993598549982516No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC124770.4085049706840305No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA104750.34295820853692555No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC99100.32445974669221306No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA98600.3228227146705571No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA98460.32236434570449346No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97240.31836998757165286No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA89330.29247214098905544No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA88980.2913262185738963No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA88470.2896564459118072No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT87800.2874628230027882No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTA86790.28415601831904314No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT75290.24650428182095585No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT74720.24463806531626803No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC71870.23530698279282902No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC65470.2143529729156326No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT65010.2128469034557091No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC65000.212814162815276No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA64880.21242127513007858No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC64700.2118319436022824No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT62730.20538203743695788No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT61980.20292648940447394No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA61300.2007001258550218No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG60820.1991285751142321No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC56570.18521380293015635No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC55840.18282373617853864No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG54440.17824004651790193No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA53570.17539161080022053No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC53560.1753588701597874No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG53370.17473679799155814No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT52350.17139725266737998No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG48630.15921773442625956No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC48400.1584646996962978No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGATGCA48010.15718781471940618No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATA47080.15414293515912605No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA44850.14684177234254042No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44390.14533570288261694No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA43510.14245452652450244No Hit
CTGACCTATGAATTGACAGCTTATGAACCGGCATG40860.1337782568097258No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40410.13230492799023544No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40260.13181381838373865No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT39820.1303732302046814No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC38630.12647709399314017No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38330.1254948747801466No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT37360.12231903265813401No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC37100.12146777600687292No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC36810.12051829743431246No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA36370.11907770925525521No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA36290.11881578413179025No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTG36130.11829193388486034No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC35780.11714601146970115No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG35710.11691682698666932No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG35620.11662216122277123No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC35400.11590186713324262No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCG34410.11266054373036381No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC33840.110794327225676No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC33240.10882988879968884No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA33050.10820781663145955No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA32930.10781492894626213No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA32110.10513019643074635No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA32060.10496649322858075No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA31680.10372234889212222No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC31380.10274012967912863No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAGCATG31300.10247820455566367No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA30950.10133228214050449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)