FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4057235
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1462323.6042280025682514No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA773331.9060517815704539No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT514351.2677352926315582No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA464041.14373458771799No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT393910.9708828795965725No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT356360.8783321646392186No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT287120.7076740686699193No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA267650.6596857219263859No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA250330.6169965506065092No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC242790.5984124656323826No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAT221440.5457904213090935No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA196100.4833340932925995No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC186200.4589332390162266No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA183190.4515143934231071No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAA160200.3948501873813077No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145600.3588650891555456No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC126300.31129574698039425No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA119790.2952503367441127No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA118060.2909863490776354No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA112960.2784162120261705No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA110250.27173678625960784No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT108940.26850798635031004No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC103310.25463154093859486No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT99040.24410713207393703No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT95610.23565309872363815No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC94280.23237500415923654No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC91200.22478362727325385No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGC89670.22101258615781438No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA88680.21857250073017706No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA87620.21595988400967656No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA81040.1997419424805317No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG80480.19836169213762575No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC80410.1981891608447625No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA79910.1969567944671679No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG74230.18295711241769333No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC72080.1776579369940366No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC72060.1776086423389328No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT69390.17102780588257765No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA69230.1706334486417474No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC68630.16915460898863388No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT67410.16614763502730306No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA64830.15978862451891498No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT64060.1578907802974193No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA63920.15754571771169282No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC63360.15616546736878686No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT63160.15567252081774904No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA61600.1518275377196539No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGG59380.1463558310031339No Hit
GCTCCCCGTCCTCCCCCCTCCCCCGCCTTGGCCGT59370.146331183675582No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA57540.1418207227335858No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA57000.14048976704578364No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT56950.1403665304080242No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC56810.1400214678222977No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA55240.13615183739665068No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG53180.13107448792096096No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52160.12856046051066797No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC51380.1266379689616204No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC50190.1237049369829453No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC49990.12321199043190745No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA48590.11976136457464259No Hit
TAGCACCATCTGATTAGCCAATGCGGAACCGGCAT45450.11202210372334855No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG44600.10992708088143772No Hit
TAGCACCATCTGAAATCGGTTACGCCTTAATTACG43910.1082264152803572No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG43540.10731446416093718No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC42850.10561379855985664No Hit
GCTCCCCGTCCTCCCCCCTCCCCGCCTTGGCCGTA42000.10351877571794584No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG41830.10309977114956367No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG41810.1030504764944599No Hit
GCTCCCCGTCCTCCCCCCTCCCGCCTTGGCCGTAC41360.10194134675462477No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG41100.10130051623827557No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC41020.10110333761786044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)