FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4721316
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1066422.258734640934858No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA798091.6903973383692175No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT692751.4672815799662637No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN638801.3530125922518212No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT606531.2846630049757315No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAC524361.110622546764504No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA523031.1078055355752505No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA371780.7874499398049187No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT362870.7685780828904484No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN312580.6620611710802666No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA304330.644587229492794No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA265810.5629998076807399No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240390.5091588870560665No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC238130.5043720860878619No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA236870.5017033386454116No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN235780.4993946603023394No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA234930.4975943148054483No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA228870.4847589104393775No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA213320.45182317811389877No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA204750.43367145939818474No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA158850.3364528025660642No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC141790.30031880941669653No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT139360.2951719393491137No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC134550.28498410189023565No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA133440.2826330624766485No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT128210.2715556425369537No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA120770.25579732430534197No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA120440.25509836664184304No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC116510.24677441628562885No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT106780.2261657554800399No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGTCTG104290.22089180220091179No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC103920.22010812239638272No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA102310.21669805621991833No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA100870.21364805914283222No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCGCCTTGGC98380.2083741058637041No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA87400.18511787815092232No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT86880.18401649031753012No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC86320.18283038034310772No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT82680.17512066550936223No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC81470.17255782074319956No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG79410.1681946304801458No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA78840.16698733997046586No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC75640.16020956868805222No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC74460.1577102655276622No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA73570.15582519788974092No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA71430.1512925633446268No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC69970.1482002051970256No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG69590.14739534485723896No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA68420.14491722223210646No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA66930.14176132247873263No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA65660.1390713945010247No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA65600.13894431128947946No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT64690.13701688258104308No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC64480.1365720913406347No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA63180.13381862175715414No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT62910.1332467473052005No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT62170.13167938769614235No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC61600.13047209718646244No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA58990.1249439774842438No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA57910.1226564796764292No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC55630.11782731763770948No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG55570.11770023442616423No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA54330.11507384805422895No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC54260.11492558430742615No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGCTCA53630.11359121058620097No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC49280.10437767774916995No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG49020.10382698383247382No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC48620.10297976242217212No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA48300.10230198529393075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)