FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780786
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1096583.9434174366528025No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA510531.8359197723233647No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT497901.7905009590813532No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT401371.4433688892277219No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN384341.3821272115150176No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT359291.2920447671989144No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA290491.044632704566263No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA289921.042582924396196No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA289761.0420075475063526No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA242580.8723432871137873No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT234610.8436823257884642No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT232950.8377127905563392No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196780.7076416523961211No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT157890.5677891071085658No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA151090.5433355892902223No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA147330.5298142323789029No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136610.4912639807593968No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA125250.4504122215805172No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121160.4357041498338958No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA101000.36320666171363064No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC99730.3586396076504988No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG88810.31937013491868843No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA87670.3152705745785544No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC76990.2768641671815091No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67920.24424748973851276No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA67130.24140656634491112No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA67010.2409750336775286No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC66580.23942870828607454No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA66190.2380262271170813No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT63010.22659061143144418No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC61870.22249105109131015No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG61540.22130433625600818No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT60410.21724073697148935No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA59820.2151190346901919No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA58700.2110913964612883No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC55890.20098633983341402No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT54950.19760600060558414No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA54550.19616755838097574No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA52280.18800439875632285No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT52270.18796843770070765No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT51950.18681768392102088No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA51550.18537924169641246No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG51190.18408464369426486No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC49100.17656878307068577No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT48700.17513034084607734No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT45260.16275973771444477No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG45030.1619326334352949No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCGCCTTGGC44250.15912767109730847No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG43720.15722173514970228No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA41550.1494181860812015No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA40450.1454624699635283No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA39740.1429092350148483No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT37500.13485395855704108No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA37470.13474607539019542No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA36510.13129381405113516No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT34700.12478486298478199No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC33800.12154836797941301No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC33360.11996608153234374No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA32300.11615420963713137No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT31930.11482365057936858No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA30920.11119158396223226No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC30700.11040044073869762No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA30560.10989698596008467No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT29870.10741567312263511No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG29590.10640876356540921No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA29430.10583338667556581No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT29320.10543781506379851No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC29230.10511416556326161No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG29060.10450282761780302No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA28670.10310034644880979No Hit
TGGCTCAGTTCAGCAGGATGTCAGCGGAACCGGCA28610.10288458011511853No Hit
CGCCTTGGCCGTACAGCAGACTCACGATAAGAGAA28020.1007628778338211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)