FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359346
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC702712.978410118736294No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT367811.5589489629753328No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN311031.3182890512879417No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC257011.0893272966322023No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAATTAC207500.8794810087202131No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN201720.854982694356826No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA197760.8381983820940209No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA175780.7450369721100678No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT175480.7437654333022795No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA174990.741688586582892No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC168340.7135028096769189No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA159500.6760347994740915No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA158820.6731526448431048No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA144740.6134750901309092No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139240.5901635453214578No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117890.49967236683385985No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA116260.492763672644877No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT110320.46758720425066946No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA101640.43079734807866243No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC99510.4217694225433658No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA92440.39180349130648917No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC82750.3507327878149284No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA80820.3425525548181572No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG74320.31500254731607824No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA73260.31050977686189307No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC70390.29834538893405205No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA65880.2792299221903019No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT62110.2632509178390961No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA59570.25248522259982215No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA59540.2523580687190433No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA53670.2274782927133197No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA52440.22226498360138786No Hit
TAGCTTATCAGACTGATGTTCTGCGGAACCGGCAT52230.22137490643593605No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC48760.2066674408925185No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48350.20492967118854125No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA47820.20268328596144863No Hit
CGTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGC47730.20230182431911214No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC46380.196579899684065No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG44630.1891625899719668No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA43030.18238104966376276No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC41120.17428558592084414No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC40200.17038620024362683No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA38400.16275696739689727No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG36510.15474627290783124No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA36230.15355950335389554No Hit
GTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGCA34970.14821904036118483No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT33400.1415646539337596No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA32740.13876726855662544No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA32310.13694472959879558No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAGCA31780.134698344371703No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT31500.1335115748177673No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC30990.13134995884452727No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA30370.12872211197509817No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC29600.12545849570177497No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT29150.12355118749009258No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA29130.12346641823624005No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC28900.12249157181693571No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGCGGCATGT28180.11943987867824388No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT28130.11922795554361251No Hit
CGCCTTGGCCGTACAGCAGCTAAGACGACAGAGAC27570.11685441643574108No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA27470.11643057016647834No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGCGGCA27440.11630341628569951No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC27190.11524380061254264No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGT27060.11469280046250103No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA26600.11274310762389238No Hit
CCCCGGCACCTCCACCACGCCTTGGCCGTACAGCA26250.11125964568147276No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA25290.1071907214965503No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT24950.10574964418105695No Hit
CTGACCTATGAATTGACAGCCACGCCTTCCGGCAT24950.10574964418105695No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG24160.10240125865388121No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC23930.10142641223457687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)