FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384769
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2843195.280059367449189No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT2023423.7576727989631498No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1729363.211576949726163No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1060331.9691281093023674No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA731881.3591669391946062No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN702031.3037328063655098No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA587691.0913931498268543No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT483200.8973458285768618No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN474130.8805020233922755No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT468750.870510879853899No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA450240.8361361462302282No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA425360.7899317500899296No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC413890.7686309292004913No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT381770.7089812023505557No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA380590.7067898362956703No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT299190.5556227203061079No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC290400.5392989002870875No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA273510.5079326522641918No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA269550.5005785763511861No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA268730.49905576265202833No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN244320.4537241987539299No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217910.404678455101788No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA200630.37258794202685386No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA183510.3407945633322432No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA174740.32450788511076334No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC171920.3192708916575623No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA165050.30651268420242356No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA150560.2796034518843798No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT147550.2740136113545446No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT146550.27215652147752295No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA133940.24873861812828No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC129870.24118026232880185No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA129480.2404559972767634No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC127790.2373175153845968No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA124640.23146768227197861No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA116910.21711237752260124No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC116350.21607240719146914No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA114590.212803929007911No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA111050.20622983084325436No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA110110.20448416635885402No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA108610.20169853154332154No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA108000.20056570671833834No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC104980.19495729528973296No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA101360.18823462993491458No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC93210.1730993474371881No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT88790.16489101018075242No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGAAAC84260.15647839303784433No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT83120.15436131057803965No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA82560.15332134024690752No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT81380.15112997419202198No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC79940.1484557647691108No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT79050.14680295477856153No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG77470.14386875277286731No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC76390.14186309570568392No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA75660.14050742009545814No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA75640.1404702782979177No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT75180.13961601695448775No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA72300.1342675981086654No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC71870.13346904946154606No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA69860.12973629880873255No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA69320.12873347027514087No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC68640.12747064915876613No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67560.12546499209158277No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG67540.12542785029404233No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG66970.12436930906414No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA66870.12418360007643782No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG66360.12323648423915678No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA65090.12087798009533929No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA64330.11946659178880283No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA63620.11814805797611745No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCAACCGGCAT62710.11645810618802774No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA62620.1162909680990958No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT62470.11601240461754254No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG61740.11465672900731676No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC60800.1129110645229164No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG60450.11226108306595882No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT60420.11220537036964817No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA59810.11107254554466497No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC59510.11051541858155846No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC58570.10876975409715813No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGAGGT57500.10678266792874494No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA56600.10511128703942546No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT56230.10442416378492744No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG55130.10238136492020362No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC55030.10219565593250148No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC54930.10200994694479931No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA54510.1012299691964502No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC53990.10026428246039895No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT53880.10006000257392657No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)