FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372628
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN318840.9453755350427026No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAC301020.8925384003216482No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286480.8494266192417308No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN244920.7261992724961068No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTATAATG192160.5697634011222109No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAATTACG181910.5393716709936583No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166500.49368029916136613No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTATAAT145300.43082130611499403No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAG138480.41059968665384977No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTATAATGC134950.40013307130226045No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTATAATGCA131040.3885397381507833No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCCGGCA125220.3712831655314491No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT122060.36191361751132944No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTATAATA108590.32197443655214864No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTATAATG105960.3141763633581883No Hit
GCCGCCGGTGAAATACCACTACTCGCCTCGCGGCA91410.2710349318098527No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCCGGCT90400.2680402344996246No Hit
GCCGCCGGTGAAATACCACTACTCGCCTCGCGGCG82960.2459802859965582No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAATG79190.23480205940293444No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTCGCGGC69710.2066934153425756No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA60640.17980044048735883No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGCCGT60430.1791777806505787No Hit
TCCCCGGCATCTCCACCACGCCTTGCGGCATGTCG57490.1704605429356573No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTATAATC55970.1659536717361061No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA55160.16355198379424No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAGCCGTA51800.15358942640575837No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTATAATC50520.14979416644824156No Hit
TCCCCGGCATCTCCACCACGCCTTGCGGCATGTCA49790.14762968225372025No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTAATTACG48430.14359721854885862No Hit
GCCGCCGGTGAAATACCACTACTCGCCTCGCGGCT47110.1396833567176694No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTTTAATG45080.1336643116287951No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT43500.1289795376187353No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTATAATGC42090.12479882157178318No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCGCCGT41480.1229901429982791No Hit
TCCCCGGCATCTCCACCACGCCTTATAATGCACTA40530.12017334849855957No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTAATTACG39580.11735655399884007No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA37950.11252352764668976No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGCGGCA36000.10674168630516025No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTCGGCCGT35640.10567426944210864No Hit
TCCCCGGCATCTCCACCACGCCTTATAATGCACTG35620.10561496850527245No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTAATTA35400.10496265820007425No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGA35350.10481440585798374No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC33840.10033718512685062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)