FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537058
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC760472.1500071528371882No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN608531.7204411123594807No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT523391.4797325913230712No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA505951.4304260772653432No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT476941.348408762310372No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN476001.3457511864379945No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAC462051.3063116296085617No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA373841.0569235788613023No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA362221.0240714175453158No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA280150.7920424262197566No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT263210.7441495163494632No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN253850.7176868459606826No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA242960.6868985467583512No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA223110.6307784605171869No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN209620.5926394195401942No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC198870.5622469295103445No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA180250.50960430957027No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT167280.4729354169482095No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA157760.44602039321944964No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA154080.435616266399929No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA126560.35781149192351386No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG126220.3568502410760581No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC120580.3409047858417928No Hit
CTGACCTATGAATTGACAGTTTATTCCAATTACGT119670.3383320262206614No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA117560.33236661654968624No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC112830.3189939209365524No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT111780.31602535214293914No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC109560.30974894955072835No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC109080.30839188953079083No Hit
CTGACCTATGAATTGACAGTTGTATTCCAATTACG108370.30638457158463334No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC100700.28469988334938245No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT96710.2734193219336522No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA94580.26739736809517967No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT87960.24868124865354202No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA83720.2366938851440943No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC82200.23239652841429234No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT82180.23233998424679494No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA81360.23002167337940177No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC79970.22609185373833282No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT79260.2240845357921753No Hit
CCACTACGCCTTGGCCGTACAGCAGCATTCAACTC75230.21269088604145026No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT73510.20782808763667432No Hit
CTGACCTATGAATTGACAGCCACGCCTTGTAATGC68420.19343759700858737No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA67830.19176954406741423No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC65120.18410780937151722No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA63480.17947118763673087No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA61740.17455184506445753No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT61410.17361886630075052No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA61130.172827247955787No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA60410.17079165792588077No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAA59790.16903878873346154No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA59590.1684733470584876No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG59150.1672293753735449No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT59090.1670597428710527No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA57220.16177286321004633No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA55780.1577016831502339No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC55130.15586399770656856No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC54000.15266925224296576No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG53610.15156664097676656No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA52370.14806090259192808No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA50840.1437352737783774No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC50240.14203894875345555No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC47660.13474475114629164No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA47280.13367041196384113No Hit
CTGACCTATGAATTGACAGTTGTATTCCATAATGC47170.13335941904260548No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC47000.1328787936188776No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC46370.13109765234270968No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT45500.128637981056573No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC43960.1242840801592736No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC43000.12156996011939868No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC42810.12103279052817341No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA42750.12086315802568123No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC42700.12072179760693774No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT41910.11848830299079065No Hit
CAACGGAATCCCAAACTACTAGATAAGGTTAATGC41210.11650925712838184No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT41090.11616999212339747No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC40050.11322969541353295No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC39110.1105721195411554No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA38450.10870616201374136No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA37390.10570932113637943No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC36790.1040129961114576No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT36780.1039847240277089No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG36710.10378681944146803No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA36530.10327792193399146No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT35910.10152505274157222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)