FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467636
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1445444.16837292034112No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1237273.568050395139513No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA917252.6451738302405445No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA535821.5452025529784557No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT528041.522766518746489No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN433651.2505637846648263No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT401071.156609286557182No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA390351.1256948537851146No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA371291.0707294537258236No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA292400.8432257595664596No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN270490.7800415037795201No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA269000.7757446283289249No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA239490.6906434239349228No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC212590.6130689611020302No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA202910.585153689718298No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA190620.5497116767734561No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC186160.536849888511943No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN183960.5305055086520039No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT168010.4845087546674449No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA166950.48145191709856516No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT156950.4526138268261144No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN156880.45241196019420726No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA146560.42265105103303807No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCC146440.42230499394976867No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA137930.39776377912791305No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT121770.35116142524763266No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA118440.3415583411869066No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA112520.3244861917456157No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA110910.31984325921175116No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA109460.3156617361222458No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT95540.2755191144629944No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA94730.27318322915092585No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA93930.2708761819291298No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTCAAA92570.2669542016520765No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC89840.25908140300769744No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC85750.24728662408626512No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA85100.2454121482185558No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA84800.2445470055103823No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT83380.24045199669169426No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT82640.23831797801153293No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT76990.22202445700759826No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG76720.2212458285702421No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT75010.21631451513365302No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC74520.21490144871030292No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC73680.21247904912741708No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT73430.21175809687060582No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG70500.20330853642077773No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA70420.20307783169859814No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC68190.1966469375678416No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA65090.18770712958338187No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG64480.18594800607676237No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG63390.18280465423706527No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT62620.18058412128608656No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT62470.18015154993199978No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC60700.17504720795377599No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA58310.16815490437866026No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA57190.16492503826814578No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG54330.15667734445022488No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA54260.15647547781831772No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA52530.15148648820118374No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC52400.15111159302764188No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT52120.15030412650001326No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51940.14978504087510916No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCA49030.141393156605826No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA47700.13755769059959003No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG47560.13715395733577573No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA46900.135250643377794No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC46700.13467388157234497No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC46080.13288591997545302No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA45190.1303193299412049No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC45060.12994443476766304No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC44210.1274931970945047No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA44200.12746435900423228No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGTGGG42760.12331167400499937No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC42690.12310980737309221No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA42080.12135068386647271No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC41170.1187264176516797No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40880.11789011303377864No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC40490.11676542751315305No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT39710.11451605647190191No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA39080.11269925678473751No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTGTTT38830.11197830452792625No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA38790.11186295216683642No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC38210.11019034293103429No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT37770.10892146695904645No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA37380.10779678143842088No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA37120.10704699109133715No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA36330.10476878195981355No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA35740.10306733463373895No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC35540.10249057282828992No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC35520.10243289664774503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)