FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929388
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1232174.206236934130952No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1026363.503666977539336No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1007643.4397628446624346No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA547751.8698444862886034No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA336151.1475093091116642No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA320591.094392412340052No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA265120.9050354545044903No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA250050.8535912620656603No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA229780.7843959216054683No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC228740.7808456920011961No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT215820.7367409165327365No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGATG147740.5043374247453735No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA141810.4840942886363978No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC134210.4581503030667156No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC123300.42090702904497457No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC121750.4156158214616842No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT120230.4104270243477477No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA109360.3733202976184787No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA102250.34904901638157865No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA97200.3318099207069873No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAC95490.32597252395380877No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC91050.31081577448941555No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC90900.31030372214264546No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT83870.28630553549068954No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC82460.2814922434310511No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75770.25865470876510727No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA72800.24851607229906042No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC70180.2395722246421437No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT67860.23165248167876704No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC65090.22219658167508027No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGATTG64970.2217869397976642No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT64340.21963631994123003No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG63820.2178612051390939No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAG63720.2175198369079139No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT62880.21465234376600162No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG62670.21393547048052358No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA61830.21106797733861132No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT60530.20663019033327099No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT58810.20075865675697452No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC58240.19881285783924832No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56390.19249754556241783No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG55620.1898690101823316No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGATTC53740.18345128743614708No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC52740.18003760512434677No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC50040.17082066288248604No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49970.17058170512066004No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA49570.16921623219593993No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA48760.16645114952338166No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA48110.1642322560207115No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC47960.16372020367394147No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC47120.1608527105320292No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC46390.15836072244441501No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT44750.15276228345306256No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT44170.15078234771221838No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA42950.14661765529182205No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC42280.14433048814291588No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA42070.14361361485743782No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41550.14183850005530166No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC40430.13801517586608533No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG39760.13572800871717916No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC38560.1316315899430188No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA38180.1303343906645347No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA37680.1286275495086346No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT36030.12299497369416411No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAGCA35510.12121985889202795No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT34950.11930819679741982No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC34930.1192399231511838No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC33770.11528005166949547No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT33460.11422181015283737No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA32620.11135431701092516No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG32020.10930610762384498No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA31860.10875991845395695No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT31820.10862337116148493No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC31680.10814545563783287No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31630.10797477152224286No Hit
CGCCTTGGCCGTACAGCAGCGCGAACCGGCATGTC31490.10749685599859082No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT31300.10684825635934878No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA31140.10630206718946072No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA30670.10469763650291461No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG30610.1044928155642066No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA29720.10145463830670434No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT29620.1011132700755243No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT29490.10066949137499029No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA29450.10053294408251827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)