FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046604
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT903012.9639887560050466No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC589591.9352367422874783No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT455571.495337103213939No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA392871.2895341829788183No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA370331.2155501666773891No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA357341.1729125281789166No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT264850.8693286032579226No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA247540.8125112420255471No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA203450.6677927292158744No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC192120.6306037804716333No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT191450.6284046105105882No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA185940.6103188993384109No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182650.5995199901267116No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT174050.5712918383879231No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA135140.4435758634860323No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA133270.4374378816544585No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA129150.42391462756564363No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA125970.4134767761087428No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC116080.3810144016091359No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGGTGT114090.3744825385905093No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA107610.3532129544896547No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT100550.3300396113180446No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG85930.2820517533621042No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA82220.2698742599957198No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC72130.23675541685102497No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG72000.23632871223171767No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG68370.22441380632336858No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT66680.2188666462723741No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC61770.20275034103546113No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT61220.20094505226146883No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC60820.19963211497129263No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG55850.18331886914085324No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATCCA55660.1826952239280195No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA54310.1782640605736748No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA53410.17530995167077834No Hit
TAGCTTATCAGACTGATGTTCTGTACCACAATCCA51130.16782620911677396No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA50610.16611939063954487No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT49780.16339504576242925No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT49710.1631652817366484No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT49230.16158975698843694No Hit
TAGCTTATCAGACTGATGTTGACATAACACCGAAC49090.16113022893687529No Hit
TAGCTTATCAGACTGATGTTCTGTACCAACCGAAC46870.15384342697639733No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA46450.1524648428217123No Hit
TAGCTTATCAGACTGATGTTGACATGGTGTTACCA46010.15102061180251847No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTAGGT45510.14937944018979823No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45420.14908402929950856No Hit
TAGCTTATCAGACTGATGTTCTGTACCACAATGGT45320.1487557949769645No Hit
TAGCTTATCAGACTGATGTTGACATGGTGTTAGGT43440.14258498971313635No Hit
TAGCTTATCAGACTGATGTTGACATGGTTGGCAAC43090.14143616958423216No Hit
TAGCTTATCAGACTGATGTTGACATAACACCGTTG42920.14087817123590726No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT42600.1398278214037663No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC42130.13828512008780924No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT42130.13828512008780924No Hit
TAGCTTATCAGACTGATGTTCTGTATTGTGGCTTG41800.13720194682341386No Hit
ATCACATTGCCAGGGATTTCCATCGCCTTGGCCGT41750.13703782966214184No Hit
TAGCTTATCAGACTGATGTTCTGTACCAACCGTTG41380.13582336266872885No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT41190.13519971745589515No Hit
TAGCTTATCAGACTGATGTTGACATGGTTGGCTTG40740.13372266300444693No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC40430.13270513660456038No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39860.13083420096605924No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA39670.13021055575322557No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA39110.12837244354697885No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT38820.12742056401160112No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG37950.12456492540546785No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37750.12390845676037976No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT36730.12056046667043042No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATTTG36690.1204291729414128No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA36300.119149059083491No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG35660.11704835941920906No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA34060.11179661025850422No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG33980.11153402280046898No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA33960.11146837593596017No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTACCA33580.11022108551029276No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA33500.10995849805225752No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC33060.1085142670330637No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG33040.10844862016855489No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT32780.10759521092994036No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG32780.10759521092994036No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31920.1047723957560615No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC31460.10326251787235885No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC31060.10194958058218266No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT30990.10171981655640182No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA30690.10073511358876966No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30500.10011146837593597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)