FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797907
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2000225.266637650684969No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1007622.6530928745753912No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN703781.8530732848382014No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT652801.7188414566233454No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT649091.709072918320538No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA607581.5997758765551657No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN484491.2756763132957178No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA413941.0899161037908511No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT360160.948311793838027No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA351660.9259310457049107No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC320440.8437278743265698No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA317300.8354601626632775No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA310990.8188457484609286No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA288150.7587073617126486No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT284490.7490704748694478No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA282880.7448312978701164No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278440.7331406482570532No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC276920.7291384438850135No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT258200.6798481374083146No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA244050.6425907743396561No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN214200.5639948529545352No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCC204600.5387177727100743No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA191880.5052256413861634No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA172890.4552244170275891No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC165120.43476578020472856No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA164680.43360724736019074No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA162230.42715632583946894No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTCAAA152970.4027744755203327No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA151090.3978243806391257No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC150280.3956916269934993No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC143820.3786822584123308No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA131940.3474018716098104No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA128920.3394501234495737No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT128880.3393448022818884No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA124860.3287600249295204No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC123690.3256793807747267No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCA122110.3215191946511592No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC117020.3081170760632106No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT112160.29532055418945224No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA110710.29150266186086177No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA110670.2913973406931765No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC109530.2883956874141468No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA107870.28402485895520874No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC104460.27504622941004087No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC104050.273966687441267No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA103440.27236053963406687No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA98230.25864245754306253No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA94190.24800501960685187No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC90360.23792051780098883No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAATTAC84270.22188537002090888No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC83760.2205425251329219No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA82950.21840977148729548No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC80240.21127426237661953No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT80050.21077398683011458No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC79040.2081146273460619No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT79010.208035636470298No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA75580.19900434634128744No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT72150.18997305621227692No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA70530.18570754892102412No Hit
CGCCTTGGCCGTACAGCAGGCACCCACAACTCAAC70040.18441736461687977No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC69960.18420672228150928No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG61510.16195762560799937No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA61220.16119404714228125No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC60960.16050945955232712No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA60480.15924560554010406No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT60420.1590876237885762No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC60090.15821872415517282No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT59770.1573761548136908No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA57590.15163615117484444No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT56790.14952972782113938No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC54410.14326311834386676No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC54030.14226256725085687No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA53970.14210458549932897No Hit
CGTACCGTGAGTAATAATGCGACGCCTTGGCCGTA52520.13828669317073852No Hit
TCAATCCCCGGCACCTCCACCACGCCTTGGCCGTA52160.13733880266157122No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT52130.1372598117858073No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG49190.12951870596094112No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA49180.1294923756690198No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA48860.12864980632753775No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG48560.12785989756989838No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG48290.1271489796880229No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG47090.12398934465746528No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA46850.12335741765135376No Hit
TAGCACCATTTGAAATCGGTCGCCTTGGCCGTACA44930.11830200160246156No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA44590.11740677167713691No Hit
CGTACCGTGAGTAATAATGCGTCGCCTTGGCCGTA43930.11566897241033021No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG43780.11527401803151051No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA43680.11501071511229738No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA42090.11082419869680853No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG41930.11040291402606751No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG41770.1099816293553265No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC40890.10766456366625092No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG40730.10724327899550988No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCA40680.10711162753590334No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA39670.10445226805185065No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT38860.10231951440622428No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA38750.10202988119508982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)