FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342780
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC750373.202904241968943No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN447771.9112763469041054No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN270821.1559770870504273No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA233060.994801048327201No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205740.8781874525136804No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG187950.8022520253715671No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC159650.6814553649937254No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT154480.6593875652003175No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA153030.653198337018414No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT148180.6324964358582539No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA136740.5836655597196493No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136330.5819155020958008No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG127330.5434996030357098No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA123090.5254014461451779No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG121970.520620800928811No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC112780.48139389955522927No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA110520.4717472404579175No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA109970.46939960218202303No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC106690.4553991411912343No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG98560.4206967790402855No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA94700.4042206267767353No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT89710.38292114496452934No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA80160.34215760762854386No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA79710.34023681267553935No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA79040.33737696241217696No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG76960.32849862129606705No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA75400.32183986545898463No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC72880.31108341372215914No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA71360.3045953952142326No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA70640.3015221232894254No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC66610.2843203373769624No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCCG65990.28167390877504506No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA61380.2619964315898206No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA61100.2608012702857289No Hit
GTCCAGTTTTCCCAGGAATCCCCCGCCTTGGCCGT60700.2590938969941693No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCCAGATTG57220.2442397493576008No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT54510.23267229530728453No Hit
GCATTGGTGGTTCAGTGGTAGAATAGAGCGGAGCG51830.2212328942538352No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCCG51710.22072068226636732No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC50730.2165376177020463No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT50570.21585466838542244No Hit
CAACGGAATCCCAAAAGCAGCCCGCCTTGGCCGTA48390.2065494839464226No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC47680.20351889635390435No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA46580.1988236198021154No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC46020.196433297193932No Hit
TCCCTGAGACCCTTTAACCTGCCGCCTTGGCCGTA45100.19250633862334493No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCG44980.19199412663587703No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCCG43790.1869146910934872No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC43490.1856341611248175No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA42570.18170720255423045No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAATA42170.17999982926267083No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC42140.17987177626580386No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGCG40440.17261543977667557No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG40250.17180443746318477No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT39770.16975558951331324No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC38750.16540178761983626No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG38150.16284072768249686No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA37800.1613467760523822No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA37520.16015161474829048No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC36970.15780397647239605No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC36900.1575051861463731No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC36770.15695028982661624No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA35850.15302333125602915No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC34960.14922442568230906No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT34260.14623652242207974No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG34250.14619383808979078No Hit
TAGCTTATCAGACTGATGTTGACGCGGTTGGCCGT33610.14346204082329542No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCCG33540.14316325049727247No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG33430.14269372284209358No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC32930.1405595062276441No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG32810.1400472942401762No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG32140.13718744397681387No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC31210.1332178010739378No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCGCC31160.13300437941249285No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGATATTTA30700.13104090012719932No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC30210.1289493678450388No Hit
CCCAGTGTTCAGACTACCTGTTCGCCTTGGCCGTA29230.12476630328071778No Hit
ACAGTAGTCTGCACATTGGTTACGCCTTGGCCGTA28860.12318698298602515No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA28370.12109545070386463No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA28010.119558814741461No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA27140.11584527783231886No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC26700.1139671672116033No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGCGCCTTGG26550.11332690222726846No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCGC26230.1119610035940208No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG26140.11157684460341988No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT25850.11033899896703916No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG25040.10688156805163096No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC24780.10577177541211723No Hit
CCCAGTGTTCAGACTACCTGTTCCGCCTTGGCCGT24210.1033387684716448No Hit
TAGCACCATTTGAAATCAGTGTCCGCCTTGGCCGT24140.10303997814562188No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT24030.10257045049044299No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC23820.1016740795123742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)