FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045465
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN598061.9637723631694994No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN374251.228876378484074No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGATGCA278950.9159520795674881No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN221190.7262930291433328No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT164660.5406727708248166No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154760.5081654197306487No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCTACG140190.4603237929183228No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT122060.4007926539953669No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATCGTAC107670.3535420699302077No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC87160.2861960324613811No Hit
TCCCCGGCATCTCCACCACGCCTTGCGGCATGTCG86530.2841273828462977No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGCGATGC85390.28038411211424197No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGGGCA66020.21678134537747107No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCTACG62910.20656944013475775No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC53220.17475163891228432No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGCGATGC52150.17123821813746012No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGGATGC48130.1580382634507374No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCCTACG48050.15777557778533No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA46520.15275171443441315No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCTACG45120.14815471528978336No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGCGGCA42900.1408651880747275No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCCTACGT42570.1397816097049219No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCTCGTA42570.1397816097049219No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT42240.1386980313351163No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC41210.13531595339299582No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA40220.13206521828357903No Hit
TCCCCGGCACCTCCACCACGCCTTGCGGCATGTCG39760.1305547757074864No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCAGCATG38260.1256294194810973No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGCCTACG36640.12031003475659711No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGGGCAT36000.11820854943333776No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG35800.11755183526981922No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGAGCAT35230.11568019990379136No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA35150.11541751423838396No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT34260.11249513621072645No Hit
CTGACCTATGAATTGACAGCCCGCGAACGCTACGT32200.10573098032648545No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG31400.10310412367241127No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCAGCA30860.1013309954309112No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG30730.10090413122462415No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)