FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724392
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC782192.871062607730459No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT592502.174797165752946No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN552052.0263236714833988No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA420541.5436104642797366No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT348181.278009919277402No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA329451.2092606350334312No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA323061.1858058605369566No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN285401.0475731833010815No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT252190.9256744257067264No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN222590.8170263310125708No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT206680.7586279801144622No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA206150.7566825919324385No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA175440.643960193687252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG173740.6377202693298174No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN159110.5840202144184831No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT134140.49236673723898766No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC134000.4918528611154342No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG125960.4623416894485082No Hit
CCACTACGCCTTGGCCGTACAGCAGCAGTTACCAT117160.4300409045394349No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA117120.4298940827898482No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA115420.4236541584324136No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA113890.4180382265107224No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA113190.4154688458929552No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC110520.4056684941080432No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC106910.3924178312078438No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG103100.3784330595597109No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT95750.3514545630731554No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC88940.32645816020602025No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC81050.29749757010004435No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT79830.2930195067376501No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA76980.2825584570795979No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT76010.27899802965212056No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGAT72070.2645360873178309No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT71870.26380197856989746No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA65600.2407876693221827No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGCG63690.23377693077941794No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA61410.2254080910529762No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA58990.21652537520298107No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA56640.20789959741476263No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG55400.20334812317757503No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA48800.17912253449577006No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA48740.17890230187139003No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG47990.17614939406663946No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA47060.17273578838874876No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA46140.16935888814825473No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA46050.16902853921168468No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA43780.16069640492263962No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT41970.1540527207538416No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA40960.1503454715767775No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC40410.14832667251996043No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG36080.1324332181272005No Hit
TCAGTGCACTACAGAACTTTGTCCGCCTTGGCCGT36080.1324332181272005No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC35330.12968031032244992No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA34760.1275881003908395No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG34610.1270375188298894No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC33690.12366061858939535No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA31760.11657646917183724No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA31660.1162094147978705No Hit
AGCACCATTTGAAATCAGTGTTCGCCTTGGCCGTA30340.11136429706150949No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG29670.10890503275593233No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC29450.1080975131332055No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA29350.10773045875923876No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT29000.10644576845035517No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT27930.10251828664891102No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC27670.1015639452765975No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC27650.10149053440180414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)