FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811478
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1685835.9962411229965165No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA981133.4897303126682835No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT598992.1305164045388225No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN489391.7406858598929105No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA412031.465528095898314No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN380271.3525626023038415No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT340331.2105020917823295No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA317921.1307931273159526No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA289931.0312369508137713No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT280030.9960241552663759No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA259950.9246026467217598No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC240550.8555997948410052No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA219140.779447678409719No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA217120.7722628453788363No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN204390.7269841698921351No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT173430.6168641547257351No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT171490.6099638695376596No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA154180.548394830050244No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC152810.5435219482421701No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150230.5343452803116367No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC128190.4559523496182435No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA121550.432334878665243No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA114790.40829058594803164No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC103830.36930753148344037No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA98580.3506340792992156No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC95870.3409950211241205No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA94800.3371891937265737No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA93940.3341303044163959No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA92640.32950640197077835No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA87850.31246909988269517No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA87730.3120422781184843No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA86780.30866327248514835No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA86390.3072761017514631No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA83880.29834841318338606No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC81430.2896341354974145No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA80380.2858994450605696No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA80000.2845478428072352No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA79040.2811332686935484No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTG78570.27946155011705587No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC77510.27569129119986No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC77100.2742329835054729No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC75350.2680084994440647No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA69870.24851697221176905No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA68940.24520910353913491No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT68670.24424875456966053No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT68340.24307499471808067No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA67410.23976712604544653No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA64700.23012806787035145No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT59990.2133753136250755No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA59510.21166802656823208No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT58060.20651059691735094No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAACA56390.20057066069874993No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA54640.19434617663734163No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC52900.18815726105628428No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC52400.18637883703873906No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC50650.1801543529773308No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGACAC49900.17748671695101295No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA49300.17535260812995868No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC48320.17186689705557004No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT47550.16912812406855043No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT46620.16582025539591633No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT45780.16283250304644034No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTC44480.15820860060082276No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA43750.15561210153520674No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT42040.1495298913952021No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC41750.14849840546502588No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG39790.1415269833162486No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA39330.139890833220107No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC38840.1381479776829127No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA38250.1360494373422093No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC37800.13444885572641863No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT37240.13245702082676797No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG36780.13082087073062637No Hit
TAGCACCATCTGAAGCTAACCTATTCCAATTACGT35680.12690833789202688No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA35630.12673049549027238No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA33840.12036373750746049No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC33470.11904770373447701No Hit
TCAGTGCACTACAGAACTTTCGCCTTGGCCGTACA33420.11886986133272251No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC32080.11410368496570132No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA32010.113854705603245No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC31900.11346345231938502No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT31800.11310776751587599No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC31580.11232526094815609No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG31260.11118706957692714No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC30250.10759465306148581No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC30200.1074168106597313No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA29460.10478474311376436No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA29400.10457133223165894No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG29150.10368212022288632No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA29070.10339757238007909No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA28860.1026506342927101No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA28560.10158357988218297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)